Cariology and Operative Dentistry, Department of Restorative Science, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
J Endod. 2010 Jun;36(6):1026-9. doi: 10.1016/j.joen.2010.02.029. Epub 2010 Apr 24.
This study investigated the use of 3.8% silver diamine fluoride (Ag[NH3]2F) as an antibacterial agent against Enterococcus faecalis biofilms and its ability to penetrate dentinal tubules by the formation of silver salts.
Biofilms were generated on membrane filter discs and subjected to 15-minute and 60-minute exposure times with 3.8% Ag(NH3)2F, saturated Ca(OH)2, 5.25% NaOCl (negative control), and 0.9% NaCl (positive control). Cleaned and shaped radicular dentin were applied with Ag(NH3)2F for 24, 48, and 72 hours. The presence of silver salts on the dentin surface was examined using low-pressure scanning electron microscopy.
Both NaOCl and Ag(NH3)2F were effective against E. faecalis biofilms, with no significant difference in reduction of microorganisms for both exposure times. Silver deposits were present on 66.5% of the radicular dentin surfaces after 72-hour application of Ag(NH3)2F as simulated interappointment dressings. Penetration of the silver deposits was observed at most 40 microm into dentinal tubules after smear layer removal.
Ag(NH3)2F has potential to be used as an antimicrobial root canal irrigant or interappointment dressing, especially in locations in which potential browning/blackening of dentin by metallic silver is not a major concern.
本研究调查了 3.8% 氟化银氨（Ag[NH3]2F）作为一种针对粪肠球菌生物膜的抗菌剂的用途及其通过形成银盐渗透牙本质小管的能力。
将生物膜在膜滤片上生成，并分别用 3.8% Ag(NH3)2F、饱和 Ca(OH)2、5.25% NaOCl（阴性对照）和 0.9% NaCl（阳性对照）进行 15 分钟和 60 分钟的暴露处理。将清洁和成形的根管牙本质用 Ag(NH3)2F 处理 24、48 和 72 小时。使用低压扫描电子显微镜检查牙本质表面上银盐的存在情况。
NaOCl 和 Ag(NH3)2F 均能有效抑制粪肠球菌生物膜，两种暴露时间对微生物减少的效果无显著差异。在模拟的预约间隙敷料中，72 小时应用 Ag(NH3)2F 后，有 66.5%的根管牙本质表面有银沉积物。在去除玷污层后，观察到银沉积物在牙本质小管内的渗透深度最大可达 40 微米。
Ag(NH3)2F 有潜力用作抗菌根管冲洗液或预约间隙敷料，尤其是在金属银可能使牙本质变色/黑化不是主要问题的部位。
