Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jun 15;878(20):1677-82. doi: 10.1016/j.jchromb.2010.04.011. Epub 2010 Apr 18.
A new liquid chromatography mass spectrometry (LC/MS) method has been developed for the qualitative and quantitative analyses of phosphatidylcholine hydroperoxides (PC-OOH) in human plasma using a synthetic hydroperoxide (1-stearoyl-2-erucoyl-PC monohydroperoxide, PC 18:0/22:1-OOH) as an internal standard. 1-Stearoyl-2-linoleoyl-PC monohydroperoxide (PC 18:0/18:2-OOH) was identified in plasma by LC/MS by comparison with an authentic standard. The calibration curves obtained for 1-palmitoyl-2-linoleoyl-PC monohydroperoxide, PC 16:0/18:2-OOH and PC 18:0/18:2-OOH were linear throughout the calibration range (0.1-1.0 pmol). The limit of detection (LOD) (S/N=3:1) was 0.01 pmol, and the limit of quantification (LOQ) (S/N=6:1) was 0.1 pmol for both PC 16:0/18:2-OOH and PC 18:0/18:2-OOH. Plasma concentrations of PC 16:0/18:2-OOH and PC 18:0/18:2-OOH were 89 and 32 nM, respectively, in a healthy volunteer.
一种新的液相色谱-质谱（LC/MS）方法已经被开发出来，用于定性和定量分析人血浆中的磷脂酰胆碱过氧化物（PC-OOH），使用合成过氧化物（1-硬脂酰-2-芥酰-PC 单过氧化物，PC 18:0/22:1-OOH）作为内标。通过与标准品比较，LC/MS 在血浆中鉴定出 1-硬脂酰-2-亚油酸酰-PC 单过氧化物（PC 18:0/18:2-OOH）。1-棕榈酰-2-亚油酸酰-PC 单过氧化物、PC 16:0/18:2-OOH 和 PC 18:0/18:2-OOH 的校准曲线在整个校准范围内均呈线性（0.1-1.0 pmol）。检测限（LOD）（S/N=3:1）为 0.01 pmol，定量限（LOQ）（S/N=6:1）为 0.1 pmol，对于 PC 16:0/18:2-OOH 和 PC 18:0/18:2-OOH 均为 0.1 pmol。在一名健康志愿者中，PC 16:0/18:2-OOH 和 PC 18:0/18:2-OOH 的血浆浓度分别为 89 和 32 nM。
