Rahimi Zohreh, Mozafari Hadi, Shahriari-Ahmadi Ali, Alimogaddam Kamran, Ghavamzadeh Ardeshir, Aznab Mozafar, Mansouri Kamran, Rezaei Mansour, Parsian Abbas
Medical Biology Research Center, Kermanshah University of Medical Sciences, Iran.
Blood Coagul Fibrinolysis. 2010 Jul;21(5):385-8. doi: 10.1097/MBC.0b013e328330e69a.
The aim of present study was to investigate the prevalence of factor V Leiden (FVL) c.1691G>A, prothrombin g.20210G>A and methylenetetrahydrofolate reductase (MTHFR) c.677C>T in deep vein thrombosis (DVT) patients and their possible association with DVT in western Iran. Eighty DVT patients with the mean age of 42.07 +/- 13.0 years including 44 women and 36 men and 100 sex-matched healthy individuals with the mean age of 37.63 +/- 13.3 years from Kermanshah Province of Iran with ethnic background of Kurd were studied for FVL c.1691G>A, prothrombin g.20210G>A and MTHFR c.677C>T by PCR-restriction fragment length polymorphism (RFLP) method using MnlI, HindIII and HinfI restriction enzymes, respectively. Prevalence of FVL was 11.4% in patients and 2% in control group. A significant association was found between FVL mutation and DVT with odds ratio (OR) of 6.3 [95% confidence interval (CI) = 1.32-30.05; P = 0.012]. The prevalence of prothrombin g.20210G>A variant in patients (3.8%) was nonsignificantly higher than control individuals (1.0%; OR 3.8; 95% CI = 0.39-37.81; P = 0.32). The prevalence of MTHFR c.677C>T in patients was 38.7% that was not statistically different from control group (44% P = 0.12). Venous thrombosis in legs was the most frequent clinical manifestation (n = 75), corresponding to 93.8% of the thromboembolism, followed by pulmonary thromboembolism (6.2%). We have, for the first time, determined the prevalence of inherited thrombophilia in a homogenous ethnic group of DVT patients and shown that FVL may be a risk factor for DVT in western Iran.
本研究的目的是调查伊朗西部深静脉血栓形成（DVT）患者中凝血因子V莱顿（FVL）c.1691G>A、凝血酶原g.20210G>A和亚甲基四氢叶酸还原酶（MTHFR）c.677C>T的患病率及其与DVT的可能关联。对来自伊朗克尔曼沙阿省、库尔德族背景、平均年龄为42.07±13.0岁的80例DVT患者（包括44名女性和36名男性）以及100名年龄匹配、平均年龄为37.63±13.3岁的健康个体，分别使用MnlI、HindIII和HinfI限制性内切酶，通过聚合酶链反应-限制性片段长度多态性（RFLP）方法研究FVL c.1691G>A、凝血酶原g.20210G>A和MTHFR c.677C>T。患者中FVL的患病率为11.4%，对照组为2%。发现FVL突变与DVT之间存在显著关联，优势比（OR）为6.3[95%置信区间（CI）=1.32 - 30.05；P = 0.012]。患者中凝血酶原g.20210G>A变异的患病率（3.8%）略高于对照个体（1.0%；OR 3.8；95% CI = 0.39 - 37.81；P = 0.32），但无统计学意义。患者中MTHFR c.677C>T的患病率为38.7%，与对照组（44%，P = 0.12）无统计学差异。腿部静脉血栓形成是最常见的临床表现（n = 75），占血栓栓塞的93.8%，其次是肺血栓栓塞（6.2%）。我们首次确定了同质种族DVT患者群体中遗传性血栓形成倾向的患病率，并表明FVL可能是伊朗西部DVT的一个危险因素。
