Department of Nanobio Materials and Electronics, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro (Oryong-dong), Buk-gu, Gwangju, Republic of Korea 500-712.
Lab Chip. 2010 Jun 7;10(11):1442-6. doi: 10.1039/b925244d. Epub 2010 Mar 16.
This paper presents a handheld mechanical cell lysis chip with ultra-sharp nano-blade arrays fabricated by simple and cost effective crystalline wet etching of (110) silicon. The ultra-sharp nano-blade array is simply formed by the undercutting of (110) silicon during the crystalline wet etching process. Cells can be easily disrupted by the silicon nano-blade array without the help of additional reagents or electrical sources. Based on the bench-top test of the proposed device, a handheld mechanical cell lysis chip with the nano-blade arrays is designed and fabricated for direct connection to a commercial syringe. The direct connection to a syringe provides rapid cell lysis, easy handling, and minimization of the lysate dead volume. The protein concentration in the cell lysate obtained by the proposed lysis chip is quantitatively comparable to the one prepared by a conventional chemical lysis method.
本文提出了一种手持式机械细胞裂解芯片，该芯片采用（110）硅的简单且经济高效的晶态湿法刻蚀制备而成，具有超锋利的纳米刀片阵列。在晶态湿法刻蚀过程中，通过（110）硅的下切，简单地形成了超锋利的纳米刀片阵列。无需额外的试剂或电源的帮助，细胞就可以很容易地被硅纳米刀片阵列破坏。基于该设备的台式测试，设计并制造了一种带有纳米刀片阵列的手持式机械细胞裂解芯片，可直接与商业注射器连接。直接与注射器连接可实现快速细胞裂解、易于操作和最小化裂解物死体积。通过所提出的裂解芯片获得的细胞裂解液中的蛋白质浓度与通过传统化学裂解方法制备的裂解液中的蛋白质浓度定量可比。
