丹参骨宝对维甲酸诱导大鼠股骨生物力学性能及骨密度的影响

Xu Bilian, Cui Liao, Chen Wenshuang, Wei Tianyou, Wu Tie

Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, China.

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2010 Apr;27(2):307-10.

This investigation was directed to the effects of DanShenGuBao on biomechanical properties and bone mineral density (BMD) of femur induced by retinoic acid (RA) in rats. Forty 4-month-old virgin female Sprague-Dawley rats were randomly divided into 5 groups(8 rats each) control group, RA group and different doses of DanShenGuBao groups. Rats in control group were given vehicle, rats in other four groups were given RA at 70 mg x kg(-1) x d(-1) in the morning and given different drugs in the afternoon at the same time. Rats in RA group were given vehicle, rats in other groups were given different doses of DanShenGuBao which contained 10 mg x kg(-1) x d(-1), 5 mg x kg(-1) x d(-1), 2.5 mg x kg(-1) x d(-1) tanshinol, respectively. All of rats were treated at 5 ml x kg(-1) by oral gavaged for 28 days. In preparation for the determination of dynamic changes in bone tissues, all rats were given subcutaneous injections of 30 mg x kg(-1) tetracycline on the 14th, 13th day and 5 mg x kg(-1) calcein on the 4th, 3rd day before death. At the experimental endpoint, the rats were sacrificed by cardiac puncture under sodium pentobarbital anesthesia. Physical parameters, BMD and biomechanical properties of femur were assessed. Compared with those in control group, the physical parameters (cross-sectional diameter, wet and dry weight), BMD and biomechanical properties (max-load, elasticity-load, break-strain, rigid coefficient and bending-energe) were significantly decreased in RA group. Compared with that in RA group, the BMD of femur was increased significantly in medium and high dose of DanShenGuBao group, but there was no significant change in physical parameters and biomechanical properties of femur. RA could decrease the physical parameters, BMD and biomechanical properties of femur. DanShenGuBao could increase BMD, but it was found with no obvious effect on physical parameters and biomechanical properties.

本研究旨在探讨丹参骨宝对维甲酸（RA）诱导的大鼠股骨生物力学性能和骨密度（BMD）的影响。将40只4月龄的雌性斯普拉格-道利处女大鼠随机分为5组（每组8只）：对照组、RA组和不同剂量的丹参骨宝组。对照组大鼠给予赋形剂，其他四组大鼠于上午给予70 mg·kg⁻¹·d⁻¹的RA，并于下午同时给予不同药物。RA组大鼠给予赋形剂，其他组大鼠分别给予含10 mg·kg⁻¹·d⁻¹、5 mg·kg⁻¹·d⁻¹、2.5 mg·kg⁻¹·d⁻¹丹参酚酸的不同剂量丹参骨宝。所有大鼠均按5 ml·kg⁻¹经口灌胃给药28天。为准备测定骨组织的动态变化，所有大鼠在处死前第14天、第13天皮下注射30 mg·kg⁻¹四环素，在第4天、第3天皮下注射5 mg·kg⁻¹钙黄绿素。在实验终点，在戊巴比妥钠麻醉下通过心脏穿刺处死大鼠。评估股骨的物理参数、骨密度和生物力学性能。与对照组相比，RA组的物理参数（横截面积、湿重和干重）、骨密度和生物力学性能（最大载荷、弹性载荷、断裂应变、刚性系数和弯曲能量）显著降低。与RA组相比，中、高剂量丹参骨宝组股骨骨密度显著增加，但股骨的物理参数和生物力学性能无显著变化。RA可降低股骨的物理参数、骨密度和生物力学性能。丹参骨宝可增加骨密度，但对物理参数和生物力学性能无明显影响。