Blackall P J, Eaves L E, Morrow C J
Animal Research Institute, Yeerongpilly, Australia.
Vet Microbiol. 1991 Mar;27(1):39-47. doi: 10.1016/0378-1135(91)90061-j.
Chromosomal DNA from Haemophilus paragallinarum was examined by restriction endonuclease analysis (REA) using the enzymes BamHI, EcoRI, HindIII, or SmaI. The enzyme SmaI had no apparent effect upon the DNA from eight representative H. paragallinarum isolates. The remaining enzymes cut the H. paragallinarum DNA to varying degrees, with the most useful patterns for distinguishing isolates being given by HindIII. The REA patterns given by HindIII were stable under both in vitro and in vivo conditions. The use of the enzyme HindIII showed that eight Australian isolates of H. paragallinarum were genetically similar. In contrast, 14 isolates of H. paragallinarum from outside Australia were markedly different from each other and the Australian isolates. A plasmid of approximately 6 kilobase pairs in size was found in one isolate of H. paragallinarum.
使用限制性内切酶BamHI、EcoRI、HindIII或SmaI，通过限制性内切酶分析（REA）对副鸡嗜血杆菌的染色体DNA进行了检测。酶SmaI对8株具有代表性的副鸡嗜血杆菌分离株的DNA没有明显影响。其余的酶对副鸡嗜血杆菌DNA有不同程度的切割，其中HindIII给出的区分分离株的模式最为有用。HindIII给出的REA模式在体外和体内条件下都是稳定的。使用酶HindIII表明，8株澳大利亚副鸡嗜血杆菌分离株在遗传上相似。相比之下，来自澳大利亚以外的14株副鸡嗜血杆菌分离株彼此之间以及与澳大利亚分离株明显不同。在一株副鸡嗜血杆菌分离株中发现了一个大小约为6千碱基对的质粒。
