Grattard F, Pozzetto B, Ros A, Gaudin O G
Laboratoire de Bactériologie-Virologie, Faculté de Médecine J. Lisfranc, Saint-Etienne, France.
J Med Microbiol. 1994 Apr;40(4):275-81. doi: 10.1099/00222615-40-4-275.
The genotypic diversity of 40 presumably epidemiologically unrelated strains of Pseudomonas aeruginosa belonging to nine different O-serotypes was analysed according to ribosomal DNA fingerprints. Ribotyping was performed with a digoxigenin-labelled DNA probe and four restriction endonucleases. Characteristic banding patterns of three to 12 bands were obtained with the different endonucleases. Among the 40 strains, eight, nine, 10 and 29 different ribotypes were differentiated with EcoRI, the combination EcoRI+HindIII, BamHI and PvuII, respectively. Poor correlations were noted between the results of serotyping and those of ribotyping. With the latter method, indices of discrimination were calculated for each enzyme from the data of the 40 unrelated strains: the values ranged from 0.678 for EcoRI to 0.979 for PvuII. Epidemiologically related samples were also tested; this enabled assessment of whether the method was able to cluster strains from a common origin with each of the enzymes tested. Ribotyping with PvuII endonuclease is proposed for screening large numbers of P. aeruginosa strains in epidemiological studies. Additional enzymes could be used to further increase the discrimination between isolates found to be indistinguishable with PvuII enzyme.
根据核糖体DNA指纹图谱分析了40株属于9种不同O血清型的铜绿假单胞菌菌株的基因型多样性，这些菌株据推测在流行病学上无关联。使用地高辛标记的DNA探针和四种限制性内切酶进行核糖分型。用不同的内切酶获得了3至12条带的特征性条带模式。在这40株菌株中，分别用EcoRI、EcoRI + HindIII组合、BamHI和PvuII区分出了8种、9种、10种和29种不同的核糖型。血清分型结果与核糖分型结果之间的相关性较差。使用后一种方法，根据40株无关联菌株的数据计算了每种酶的鉴别指数：值范围从EcoRI的0.678到PvuII的0.979。还对流行病学相关样本进行了检测；这使得能够评估该方法是否能够将来自共同来源的菌株与所测试的每种酶聚类在一起。建议在流行病学研究中使用PvuII内切酶进行核糖分型以筛选大量铜绿假单胞菌菌株。可以使用其他酶来进一步增加与PvuII酶无法区分的分离株之间的鉴别力。
