Mamtseva G I, Fedorov N A, Ovcharuk I N
Vestn Akad Med Nauk SSSR. 1991(2):45-6.
Storage of anticoagulated whole blood leads to the activation of platelets and granulocytes, formation of aggregates, lysis of cells, various thrombogenic substances, including proteolytic enzymes. Transfusion of the blood may cause capillary occlusion, intravascular coagulation, renal and hepatic dysfunction. Gel-silicagel affinity+ chromatography of the anticoagulated whole blood was shown to result in selective sorption of plasma fibrin. Preactivated platelets and granulocytes were also removed by interaction of cell fibronectin receptors with immobilized fibronectin. The residual platelets and granulocytes lost their ability to storage-induced activation and remained viable for at least 48 hours at 4 degrees C.
抗凝全血的储存会导致血小板和粒细胞活化、聚集体形成、细胞裂解以及包括蛋白水解酶在内的各种促血栓形成物质的产生。输血可能会导致毛细血管阻塞、血管内凝血、肾和肝功能障碍。已证明，抗凝全血的凝胶 - 硅胶亲和色谱法可导致血浆纤维蛋白的选择性吸附。预活化的血小板和粒细胞也通过细胞纤连蛋白受体与固定化纤连蛋白的相互作用而被去除。残留的血小板和粒细胞失去了储存诱导活化的能力，并在4℃下至少存活48小时。
