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[免疫酶法、比浊法和亲和色谱法测定人血浆中纤连蛋白水平(结果的可比性以及血液储存条件和时间的影响)]

[Immunoenzyme, turbidimetric and affinity chromatographic methods of determining the fibronectin level in human plasma (comparability of results and effect of conditions and duration of blood storage)].

作者信息

Gamzatova P A, Korshunova T Iu, Klemiashov G P, Ovcharuk I N, Fedorov N A

出版信息

Vopr Med Khim. 1988 Mar-Apr;34(2):123-5.

PMID:3041669
Abstract

Immunoenzymatic, turbidimetric and affinity chromatography methods used for estimation of fibronectin concentration exhibited the similar results but the turbidimetric procedure was more simple and less time-consuming. Concentration of fibronectin was not decreased in blood maintained in polymer container "Gemacon 500" within 48 hrs both at 4 degrees and 20 degrees, while about 40 = 50% of fibronectin was lost after storage of blood in glass flasks under similar conditions.

摘要

用于评估纤连蛋白浓度的免疫酶法、比浊法和亲和色谱法结果相似,但比浊法操作更简单且耗时更少。在4℃和20℃下,保存在“Gemacon 500”聚合物容器中的血液在48小时内纤连蛋白浓度未降低,而在类似条件下血液储存在玻璃瓶中后约40%至50%的纤连蛋白会丢失。

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