Section of Neurology, Department of Medical and Surgical Sciences of Communication and Behaviour, University of Ferrara, Ferrara, Italy.
Mult Scler. 2010 Jul;16(7):883-7. doi: 10.1177/1352458510368051. Epub 2010 May 18.
Cerebrospinal fluid and serum levels and intrathecal synthesis of anti-Epstein-Barr virus (EBV) IgG were measured by enzyme-linked immunosorbent assay in 80 relapsing-remitting multiple sclerosis patients grouped according to clinical and magnetic resonance imaging (MRI) evidence of disease activity. Eighty patients with other inflammatory neurological disorders (OIND) and 80 patients with non-inflammatory neurological disorders (NIND) served as neurological controls. Cerebrospinal fluid concentrations were higher in OIND than in multiple sclerosis (p < 0.0001) and NIND (p < 0.01) for anti-viral-capsid-antigen (anti-VCA) IgG, in multiple sclerosis than in NIND (p < 0.01) and in OIND than in NIND (p < 0.05) for anti-EBV nuclear antigen-1 (EBNA-1) IgG. Serum levels were more elevated in OIND than in multiple sclerosis (p < 0.05) and in MRI inactive than in MRI active multiple sclerosis (p < 0.0001) for anti-VCA IgG, and in multiple sclerosis than in OIND and NIND (p < 0.01) for anti-EBNA-1 IgG. Serum titres of anti-VCA and anti-EBNA-1 IgG were also positively (p < 0.05) and inversely (p < 0.001) correlated, respectively, with the Expanded Disability Status Scale. An intrathecal IgG production of anti-VCA and anti-EBNA-1 IgG, as indicated by Antibody Index, was present only in a limited number of multiple sclerosis patients and controls (range from 1.3 to 6.3%). These findings do not support a direct pathogenetic role of EBV-targeted humoral immune response in multiple sclerosis.
80 例复发性缓解型多发性硬化症患者根据临床和磁共振成像（MRI）疾病活动证据分组，通过酶联免疫吸附试验测量脑脊液和血清中抗 Epstein-Barr 病毒（EBV）IgG 水平和鞘内合成。80 例其他炎症性神经疾病（OIND）和 80 例非炎症性神经疾病（NIND）患者作为神经对照组。抗病毒衣壳抗原（抗 VCA）IgG 脑脊液浓度在 OIND 中高于多发性硬化症（p<0.0001）和 NIND（p<0.01），在多发性硬化症中高于 NIND（p<0.01）和 OIND 中高于 NIND（p<0.05）抗 EBV 核抗原-1（EBNA-1）IgG。OIND 中血清水平高于多发性硬化症（p<0.05）和 MRI 不活跃高于 MRI 活跃多发性硬化症（p<0.0001）抗 VCA IgG，多发性硬化症中高于 OIND 和 NIND（p<0.01）抗 EBNA-1 IgG。抗 VCA 和抗 EBNA-1 IgG 的血清滴度也分别呈正相关（p<0.05）和负相关（p<0.001）与扩展残疾状况量表相关。抗 VCA 和抗 EBNA-1 IgG 的鞘内 IgG 产生，如抗体指数所示，仅在少数多发性硬化症患者和对照组中存在（范围为 1.3 至 6.3%）。这些发现不支持 EBV 靶向体液免疫反应在多发性硬化症中的直接致病作用。
