基于微球的多重分支 DNA 分析方法的建立用于检测和区分禽流感病毒株。
Development of microsphere-based multiplex branched DNA assay for detection and differentiation of avian influenza virus strains.
机构信息
Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio 44691, USA.
出版信息
J Clin Microbiol. 2010 Jul;48(7):2575-7. doi: 10.1128/JCM.01979-09. Epub 2010 May 19.
Abstract
We developed and evaluated a multiplex branched DNA assay for the detection and subtyping of avian influenza (AI) virus strains. The assay successfully detected all 94 AI virus strains of 15 different hemagglutinin (HA) subtypes tested while simultaneously differentiating 24 North American H5, 11 Eurasian H5, and 11 H7 strains. Our study demonstrates for the first time that a branched DNA method can detect targets that show a great amount of sequence variation.
摘要
我们开发并评估了一种用于检测和亚型分析禽流感（AI）病毒株的多重分支 DNA 检测方法。该方法成功检测了 94 株不同血凝素（HA）亚型的 15 种 AI 病毒株，同时区分了 24 株北美 H5、11 株欧亚 H5 和 11 株 H7 病毒株。本研究首次证明了分支 DNA 方法能够检测到具有大量序列变异的靶标。
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