Department of Pediatrics, Mount Sinai School of Medicine, New York, New York 10029, USA.
Ann Allergy Asthma Immunol. 2010 May;104(5):426-33. doi: 10.1016/j.anai.2010.03.012.
An inactivated influenza vaccine produced in canine kidney cells (MDCK 33016-PF) contains no egg proteins and may be used to immunize egg-allergic patients. Although no major dog allergens were identified in MDCK 33016-PF cells, minor dog allergens might be present and cause reactions in dog-allergic individuals.
To evaluate the allergenicity of the inactivated influenza vaccine produced in cell culture in a mediator release assay.
Rat basophil leukemia (RBL) cells transfected with human IgE receptor-1 were sensitized with sera from dog-allergic adults with positive skin prick test reactions to dog extract and detectable dog dander IgE and were stimulated with serial dilutions of vaccine and dog dander extract. N-hexosaminidase release (NHR) was used as a marker of RBL cell degranulation. Western blots were performed, and UniCAP was used to measure dog-specific IgE antibody levels.
The median (interquartile range) level of dog dander IgE was 8.31 kU(A)/L (1.895-14.5 kU(A)/L) and of dog epithelium IgE was 3.19 kU(A)/L (0.835-6.27 kU(A)L). Median (range) maximum NHR (at the first 10-fold dilution) was 0% (0%-1.4%) to vaccine and 10.2% (0%-35.9%) to dog dander (P < .001). In an egg-allergic control subject, the maximum NHR to a vaccine cultured in chick embryo and containing egg protein was 10.2%. IgE antibodies in pooled sera did not bind to vaccine on immunoblots but produced strong binding to dog dander and epithelium extracts. Serum from an egg-allergic control subject strongly bound embryonated egg-derived vaccine.
An influenza vaccine produced in continuous canine kidney cells did not trigger degranulation in RBL cells passively sensitized with human anti-dog IgE.
在犬肾细胞（MDCK33016-PF）中生产的灭活流感疫苗不含鸡蛋蛋白，可用于免疫鸡蛋过敏患者。虽然在 MDCK33016-PF 细胞中未鉴定出主要的狗过敏原，但可能存在次要的狗过敏原，并在狗过敏个体中引起反应。
通过介质释放测定评估细胞培养中生产的灭活流感疫苗的变应原性。
用狗提取物和可检测的狗皮屑 IgE 阳性皮肤点刺试验反应的狗过敏成人的血清对转染人 IgE 受体-1 的大鼠嗜碱性白血病（RBL）细胞进行致敏，并对疫苗和狗皮屑提取物的系列稀释液进行刺激。N-己糖胺酶释放（NHR）用作 RBL 细胞脱粒的标志物。进行 Western 印迹，并使用 UniCAP 测量狗特异性 IgE 抗体水平。
狗皮屑 IgE 的中位数（四分位距）水平为 8.31 kU（A）/L（1.895-14.5 kU（A）/L），狗上皮 IgE 水平为 3.19 kU（A）/L（0.835-6.27 kU（A）/L）。最大 NHR（在第一个 10 倍稀释时）的中位数（范围）为 0%（0%-1.4%）至疫苗和 10.2%（0%-35.9%）至狗皮屑（P<.001）。在鸡蛋过敏对照受试者中，含鸡蛋蛋白的鸡胚培养的疫苗的最大 NHR 为 10.2%。免疫印迹上，混合血清中的 IgE 抗体未与疫苗结合，但与狗皮屑和上皮提取物产生强烈结合。来自鸡蛋过敏对照受试者的血清强烈结合胚胎鸡蛋衍生的疫苗。
在连续的犬肾细胞中生产的流感疫苗不会触发被动致敏的 RBL 细胞脱粒。
