Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
Ann Allergy Asthma Immunol. 2010 May;104(5):434-9. doi: 10.1016/j.anai.2010.03.008.
Rhinovirus and fungi are common environmental factors able to induce airway inflammation. They are associated with the production of chemical mediators by direct activation of epithelial cells.
To evaluate the effect of fungal stimulation of rhinovirus-infected nasal polyp epithelial cells (NPECs) on the activation and migration of eosinophils.
Rhinovirus-infected NPECs were stimulated with Alternaria and Aspergillus for 48 hours. Then, epithelial cells were co-cultured with freshly isolated eosinophils. An eosinophil migration study was performed with epithelial cell-conditioned media. Interleukin 6, interleukin 8, granulocyte-macrophage colony-stimulating factor, and tumor necrosis factor levels were measured to determine the activation of cells.
Airborne fungi enhanced the production of cytokines from rhinovirus 16-infected NPECs compared with fungi stimulation or rhinovirus 16 infection alone. Rhinovirus 16-infected NPECs were co-cultured with eosinophils, and cytokine production was not significantly increased except tumor necrosis factor-alpha production by Aspergillus. Epithelial cell-conditioned media, which were stimulated with fungi, enhanced the migration of eosinophils.
There was some synergism between rhinovirus 16 infection and airborne fungal exposure, enhancing the inflammatory response of airway epithelial cells.
鼻病毒和真菌是常见的环境因素，能够诱导气道炎症。它们通过直接激活上皮细胞与化学介质的产生有关。
评估真菌刺激鼻息肉上皮细胞（NPECs）中鼻病毒感染对嗜酸性粒细胞激活和迁移的影响。
用交链孢霉和曲霉刺激鼻病毒感染的 NPECs48 小时。然后，用上皮细胞条件培养基将上皮细胞与新分离的嗜酸性粒细胞共培养。进行上皮细胞条件培养基的嗜酸性粒细胞迁移研究。测量白细胞介素 6、白细胞介素 8、粒细胞-巨噬细胞集落刺激因子和肿瘤坏死因子的水平以确定细胞的激活情况。
与真菌刺激或单纯鼻病毒 16 感染相比，空气传播真菌增强了鼻病毒 16 感染的 NPECs 产生细胞因子的能力。鼻病毒 16 感染的 NPECs 与嗜酸性粒细胞共培养，除了曲霉产生的肿瘤坏死因子-α外，细胞因子的产生没有明显增加。用真菌刺激的上皮细胞条件培养基增强了嗜酸性粒细胞的迁移。
鼻病毒 16 感染与空气传播真菌暴露之间存在一定的协同作用，增强了气道上皮细胞的炎症反应。
