Department of Otorhinolaryngology, Catholic University of Daegu School of Medicine, Daegu, Republic of Korea.
Int Immunopharmacol. 2011 Nov;11(11):1691-6. doi: 10.1016/j.intimp.2011.05.028. Epub 2011 Jun 15.
Nano-silver is used for its anti-bacterial, anti-viral and anti-fungal properties. The aim of this study was to investigate the potential clinical efficacy of nano-silver for its anti-inflammatory effect on respiratory epithelial cell inflammation. Primary nasal polyp epithelial cells (NPECs) were exposed to Alternaria alternata, Der P1, and staphylococcal enterotoxin B for 48 h with or without various concentration of nano-silver, then the supernatants were collected. Cell cytotoxicities were measured using a CellTiter-96® aqueous cell proliferation assay kit. The interleukin (IL)-6, IL-8 and granulocyte-macrophage colony stimulating factor were measured to evaluate the inflammatory effects on the epithelial cells. Nuclear factor-κB (NF-κB) and activator protein-1 (AP-1) were analyzed using western blot and ELISA method. Cell survival was found to be significantly decreased at nano-silver concentrations exceeding 10 ppm. Alternaria, Der P1 and SEB activated NPECs with increased cytokine production. Alternaria induced NPECs not inhibited by nano-silver. However, Der P1 and SEB induced cytokine production was significantly affected by concentrations over 1 ppm. Alternaria, Der P1 and SEB enhanced nuclear NF-κB expression and nano-silver inhibited NF-κB expression in SEB and Der P1 treated group. Although nano-silver is cytotoxic at higher concentrations, at safe concentrations it can inhibit the activation of NPECs. This finding suggests a novel pharmacological rationale for the treatment of airway inflammation and/or immunological disease.
纳米银因其抗菌、抗病毒和抗真菌特性而被使用。本研究旨在探讨纳米银的抗炎作用对呼吸道上皮细胞炎症的潜在临床疗效。将原代鼻息肉上皮细胞（NPECs）暴露于Alternaria alternata、Der P1 和葡萄球菌肠毒素 B 中 48 小时，同时加入或不加入不同浓度的纳米银，然后收集上清液。使用 CellTiter-96® 水溶性细胞增殖检测试剂盒测量细胞毒性。测量白细胞介素（IL）-6、IL-8 和粒细胞-巨噬细胞集落刺激因子以评估对上皮细胞的炎症影响。使用 Western blot 和 ELISA 方法分析核因子-κB（NF-κB）和激活蛋白-1（AP-1）。发现纳米银浓度超过 10ppm 时细胞存活率明显下降。Alternaria、Der P1 和 SEB 激活 NPECs 导致细胞因子产生增加。纳米银不能抑制 Alternaria 诱导的 NPECs。然而，超过 1ppm 的浓度会显著影响 Der P1 和 SEB 诱导的细胞因子产生。Alternaria、Der P1 和 SEB 增强了核 NF-κB 表达，纳米银抑制了 SEB 和 Der P1 处理组中 NF-κB 的表达。虽然纳米银在较高浓度下具有细胞毒性，但在安全浓度下，它可以抑制 NPECs 的激活。这一发现为气道炎症和/或免疫性疾病的治疗提供了新的药理学依据。
