Laboratory of Clinical Immunology, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu, China.
Biomed Environ Sci. 2010 Feb;23(1):42-7. doi: 10.1016/S0895-3988(10)60030-X.
Elderly patients with obstructive sleep apnea/hypopnea syndrome (OSAHS) has a higher risk of cardiovascular and cerebrovascular disease. However, changes of homocysteine (Hcy) as markers of cardiovascular and cerebrovascular disease associated with OSAHS and their mechanism have not been elucidated so far. This study aims to investigate the changes of both serum Hey and oxidative stress and their possible links with OSAHS in elderly patients.
Based on polysomnogram (PSG) and age, 83 patients with OSAHS were recruited and divided into elderly-OSAHS (n=32) and non-elderly OSAHS groups (n=51). Fifty two subjects without OSAHS were divided into elderly control (n=29) and non-elderly control groups (n=23). A total of 135 subjects were included in the present study. All subjects were recorded for PSG variables and the contents of homocysteine (Hcy), malonaldehyde (MDA), and glutathione (GSH) which were detected after sleep. Serum homocysteine was measured by cyclophorase. MDA and GSH were measured by spectrophotometer.
(1) The serum levels of Hcy showed significant difference among the four groups (P < 0.05). The concentrations of Hey in elderly OSAHS patients were higher than in other groups, while those in the elderly control group were higher than in the non-elderly control; the concentrations in the non-elderly OSAHS group were higher than in the non-elderly control. (2) The concentrations of MDA and GSH changed at an equal pace with Hey in the four groups. (3) Multielement linearity regression analysis indicated a statistically significant relationship between Hcy concentration and age, MDA, GSH, and apnea hypopnea index (AHI).
(1) The concentrations of Hey and oxidative stress have increased with advancing age. (2) The concentrations of Hey and oxidative stress have further increased in the elderly patients with OSAHS. (3) Oxidative stress might cause high-level serum Hey in the elderly patients with OSAHS.
患有阻塞性睡眠呼吸暂停低通气综合征（OSAHS）的老年患者发生心脑血管疾病的风险更高。然而，目前尚不清楚同心血管疾病和脑血管疾病相关的高半胱氨酸（Hcy）标志物变化及其与 OSAHS 的关系及其机制。本研究旨在探讨老年 OSAHS 患者血清 Hcy 和氧化应激的变化及其可能的联系。
根据多导睡眠图（PSG）和年龄，招募了 83 例 OSAHS 患者，并将其分为老年 OSAHS 组（n=32）和非老年 OSAHS 组（n=51）。52 例无 OSAHS 的受试者分为老年对照组（n=29）和非老年对照组（n=23）。共有 135 例受试者纳入本研究。所有受试者均记录 PSG 变量，并在睡眠后检测同型半胱氨酸（Hcy）、丙二醛（MDA）和谷胱甘肽（GSH）的含量。血清同型半胱氨酸采用环化酶法测定。MDA 和 GSH 采用分光光度计法测定。
（1）四组血清 Hcy 水平差异有统计学意义（P<0.05）。老年 OSAHS 患者 Hcy 浓度高于其他组，老年对照组高于非老年对照组；非老年 OSAHS 组高于非老年对照组。（2）四组 MDA 和 GSH 浓度与 Hcy 同步变化。（3）多元线性回归分析表明，Hcy 浓度与年龄、MDA、GSH 和呼吸暂停低通气指数（AHI）呈统计学显著相关。
（1）Hey 和氧化应激的浓度随年龄增长而增加。（2）老年 OSAHS 患者 Hcy 和氧化应激的浓度进一步增加。（3）氧化应激可能导致老年 OSAHS 患者血清中高水平的 Hcy。
