Institute of Fluorescence, University of Maryland Baltimore County, 701 East Pratt Street, Baltimore, Maryland 21202, USA.
Langmuir. 2010 Jul 20;26(14):12371-6. doi: 10.1021/la101801n.
Multilayers of nanoburger structures of silver island films-SiO(2)-silver island films (SIFs-SiO(2)-SIFs) were used as substrates to study the fluorescence of close-proximity fluorophores. Compared to single-layered SIFs, multilayer nanoburgers exhibit several distinctive properties including a significantly enhanced fluorescence intensity, decreased lifetimes, and increased fluorphore photostability by simply varying the dielectric layer thickness while the SIF layer is kept constant. Finite-difference time-domain (FDTD) calculations show that the maximum electric field intensity can be tuned by varying the distance between the silver particles. Enhanced fluorescence emission coupled with a reduced fluorophore lifetime suggests that both an electric field and plasmon-coupling component are the underlying mechanisms for nanoburger-based metal-enhanced fluorescence (MEF). This tunable multilayer nanoburger structure holds great potential for applications in biology, microscopy, imaging, and biomedical research, given the current uses of MEF.
多层银岛膜-二氧化硅-银岛膜（SIFs-SiO2-SIFs）纳米汉堡结构被用作基底来研究近场荧光体的荧光。与单层 SIFs 相比，多层纳米汉堡结构表现出一些显著的性质，包括通过简单地改变介电层厚度而保持 SIF 层不变，就可以显著增强荧光强度、降低荧光寿命和提高荧光体的光稳定性。有限差分时域（FDTD）计算表明，通过改变银颗粒之间的距离可以调节最大电场强度。增强的荧光发射伴随着荧光寿命的缩短表明，电场和等离子体耦合分量都是基于纳米汉堡结构的金属增强荧光（MEF）的潜在机制。鉴于 MEF 的当前用途，这种可调谐的多层纳米汉堡结构在生物学、显微镜、成像和生物医学研究等领域具有很大的应用潜力。
