Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University, Nanjing 210096, P.R. China.
Chemphyschem. 2011 Apr 4;12(5):992-8. doi: 10.1002/cphc.201000849. Epub 2011 Feb 23.
A new and simple procedure to enhance the fluorescence of analytes on the surfaces of a solid substrate is demonstrated based on Ag@SiO(2) nanoparticles. Two kinds of silver-silica core-shell nanoparticles with shell thicknesses of around 3 and 15 nm have been prepared and used as enhancing agents, respectively. By simply pipetting drops of the enhancing agents onto substrate surfaces with Rose Bengal monolayers, an enhancement of about 27 times, compared with the control sample, is achieved by using the Ag@SiO(2) nanoparticles with shells of about 3 nm, whereas an enhancement of around 11.7 times is obtained when using those with thicker shells. The effects of shell thickness and surface density of the enhancing agents on the enhancement have been investigated experimentally. The results show that this method can be potentially helpful in fluorescence-based surface analysis.
基于 Ag@SiO(2) 纳米粒子，展示了一种新的、简单的方法，可增强固体基底表面分析物的荧光。已经制备了两种具有约 3nm 和 15nm 壳厚度的银-硅核壳纳米粒子，并分别用作增强剂。通过简单地将增强剂滴在带有 Rose Bengal 单层的基底表面上，使用壳厚约为 3nm 的 Ag@SiO(2)纳米粒子可实现约 27 倍的增强，而使用壳厚较大的纳米粒子则可实现约 11.7 倍的增强。实验研究了壳厚度和增强剂表面密度对增强的影响。结果表明，该方法在基于荧光的表面分析中可能具有潜在的帮助。
