College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea.
Tissue Eng Part C Methods. 2010 Dec;16(6):1481-91. doi: 10.1089/ten.TEC.2010.0149.
Mesenchymal stem cells (MSCs) offer a great promise for regenerative medicine. Present study compared the characterization of porcine MSCs (pMSCs) derived from bone marrow extract with adult ear and fetal skin-derived cells on morphology, cell growth, alkaline phosphatase activity, proliferation ability, expression of cluster of differentiation (CD) markers (CD29, 45, and 90), cell cycle, protein and mRNA levels of Oct-4, Sox-2, and Nanog, and lineage differentiation ability. Skin-derived cells exhibited alkaline phosphatase activity and differentiation ability like pMSCs. pMSCs possessed a longer doubling time than skin-derived cells, and there was no difference in the ratio of G0/G1 phase between pMSCs and skin-derived cells. Except for CD29 and 90, all cells were found negative for CD45. Protein and mRNA expression of Oct-4, Sox-2 and Nanog were observed with similar intensity in all cells. Taken together, pMSCs and skin-derived cells revealed similar characteristics, and suggested the possible supportive role of skin-derived cells with MSCs for the regeneration of damaged tissues in cell-based therapies.
间充质干细胞（MSCs）为再生医学带来了巨大的希望。本研究比较了来源于骨髓提取物的猪 MSCs（pMSCs）与成年耳朵和胎儿皮肤来源的细胞在形态、细胞生长、碱性磷酸酶活性、增殖能力、分化群（CD）标志物（CD29、45 和 90）表达、细胞周期、Oct-4、Sox-2 和 Nanog 的蛋白和 mRNA 水平以及谱系分化能力方面的特征。皮肤来源的细胞表现出与 pMSCs 相似的碱性磷酸酶活性和分化能力。pMSCs 的倍增时间长于皮肤来源的细胞，pMSCs 和皮肤来源的细胞之间 G0/G1 期的比例没有差异。除了 CD29 和 90，所有细胞均为 CD45 阴性。所有细胞的 Oct-4、Sox-2 和 Nanog 的蛋白和 mRNA 表达强度相似。综上所述，pMSCs 和皮肤来源的细胞表现出相似的特征，提示皮肤来源的细胞可能与 MSCs 一起在基于细胞的治疗中支持受损组织的再生。
