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从堕胎组织和胎盘中分离并个体化出胎儿和母体组织,用于强奸案和弃婴案件中的亲子鉴定。

Isolation and individualization of conceptus and maternal tissues from abortions and placentas for parentage testing in cases of rape and abandoned newborns.

作者信息

Johnson Donald J, Matthies Learden K, Roberts Katherine A, Yorker Beatrice Crofts

机构信息

School of Criminal Justice and Criminalistics, California State University at Los Angeles, Los Angeles, CA 90032-8163, USA.

出版信息

J Forensic Sci. 2010 Nov;55(6):1430-6. doi: 10.1111/j.1556-4029.2010.01433.x.

DOI:10.1111/j.1556-4029.2010.01433.x
PMID:20487164
Abstract

Abortion specimens are often submitted to forensic laboratories as the only piece of physical evidence in rape and incest cases. The recovery of conceptus tissues from this evidence permits the use of paternity testing to evaluate suspects. In cases of abandoned newborns, the recovery of maternal tissue from the placenta allows for the direct comparison of genetic profiles between the suspected mother and the biological mother. We report on the identification and isolation of conceptus tissues from embryonic- and fetal-period abortions, and maternal tissues from delivered placentas, by gross and low-magnification examination with manual dissection. Hundreds of single-source samples have been successfully recovered by this method and short tandem repeat typed using standard forensic procedures. We additionally describe extraembryonic tissues that can be recovered and typed in the absence of the embryo proper. We conclude that an expertise and protocols can be developed by forensic laboratories for the routine analysis of this evidence.

摘要

堕胎标本常作为强奸和乱伦案件的唯一物证被提交到法医实验室。从该证据中获取胎儿组织可用于亲子鉴定以评估嫌疑人。在弃婴案件中,从胎盘中获取母体组织可直接比较疑似母亲和生物学母亲的基因图谱。我们报告了通过大体和低倍检查及手工解剖从胚胎期和胎儿期堕胎标本中鉴定和分离胎儿组织,以及从分娩后的胎盘中获取母体组织的方法。通过这种方法已成功获取数百个单源样本,并使用标准法医程序进行短串联重复序列分型。我们还描述了在没有完整胚胎的情况下可获取并分型的胚外组织。我们得出结论,法医实验室可以开发专业技术和方案来对这类证据进行常规分析。

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