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刑事亲子鉴定案件中福尔马林固定石蜡包埋(FFPE)流产胎儿组织的短串联重复序列分型

STR typing of formalin-fixed paraffin embedded (FFPE) aborted foetal tissue in criminal paternity cases.

作者信息

Reshef Ayeleth, Barash Mark, Voskoboinik Lev, Brauner Paul, Gafny Roni

机构信息

Forensic Biology Laboratory, Division of Indentification and Forensic, Israel Police National Headquarters, Jerusalem 91906, Israel.

出版信息

Sci Justice. 2011 Mar;51(1):19-23. doi: 10.1016/j.scijus.2010.09.001. Epub 2010 Oct 14.

DOI:10.1016/j.scijus.2010.09.001
PMID:21334577
Abstract

Sexual assault or rape cases occasionally result in unwanted pregnancies. In almost all such cases the foetus is aborted. A forensic laboratory may receive the foetus, the placenta, or paraffin embedded abortion material for paternity testing. Obtaining a foetal profile DNA from a foetus or placenta may not be successful due to the age or condition of the tissue. Moreover, maternal contamination of placental material will invariably result in a mixed DNA profile. However, the use of properly screened abortion material from paraffin blocks will almost always result in obtaining a foetal DNA profile. Furthermore, foetal tissue fixed in paraffin blocks does not require special conditions for submission and storage as required to preserve fresh foetal or placental tissue. As hospitals routinely prepare foetal tissue in paraffin blocks, which should be readily obtainable by forensic laboratories, these samples would appear to be the preferred choice for paternity testing.

摘要

性侵犯或强奸案件偶尔会导致意外怀孕。在几乎所有此类案件中,胎儿都会被堕胎。法医实验室可能会收到胎儿、胎盘或石蜡包埋的堕胎材料用于亲子鉴定。由于组织的年龄或状况,从胎儿或胎盘中获取胎儿DNA图谱可能不会成功。此外,胎盘材料的母体污染将不可避免地导致混合DNA图谱。然而,使用来自石蜡块的经过适当筛选的堕胎材料几乎总能获得胎儿DNA图谱。此外,固定在石蜡块中的胎儿组织不需要像保存新鲜胎儿或胎盘组织那样的特殊提交和储存条件。由于医院通常会将胎儿组织制备成石蜡块,法医实验室应该很容易获得这些石蜡块,因此这些样本似乎是亲子鉴定的首选。

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STR typing of formalin-fixed paraffin embedded (FFPE) aborted foetal tissue in criminal paternity cases.刑事亲子鉴定案件中福尔马林固定石蜡包埋(FFPE)流产胎儿组织的短串联重复序列分型
Sci Justice. 2011 Mar;51(1):19-23. doi: 10.1016/j.scijus.2010.09.001. Epub 2010 Oct 14.
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