过氧化氢在二喹啉甲酸蛋白质测定中的潜在干扰。

Potential interference of hydrogen peroxide in the 2,2'-bicinchoninic acid protein assay.

作者信息

Baker W L

机构信息

Chemistry Department, Swinburne Institute of Technology, Hawthorn, Victoria, Australia.

出版信息

Anal Biochem. 1991 Jan;192(1):212-4. doi: 10.1016/0003-2697(91)90209-c.

DOI:10.1016/0003-2697(91)90209-c

PMID:2048723

Abstract

At a concentration of 20-800 nmol/0.1 ml hydrogen peroxide instantly reacts with a 2,2'-bicinchoninic acid copper color reagent. It also reacts with a reformulated reagent at pH 7 but the color develops less rapidly. While the effect may interfere with protein estimations at alkaline pH, the effect of pH 7 may be used to determine the possible extent of hydrogen peroxide interference after treatment with catalase.

摘要

在浓度为20 - 800纳摩尔/0.1毫升时，过氧化氢会立即与2,2'-联喹啉铜酸显色试剂发生反应。它也会与pH值为7的重新配制的试剂发生反应，但显色速度较慢。虽然这种影响可能会干扰碱性pH条件下的蛋白质测定，但pH值为7时的这种影响可用于确定用过氧化氢酶处理后过氧化氢干扰的可能程度。