一种使用二喹啉甲酸的铜定量检测方法。

A quantitative test for copper using bicinchoninic acid.

作者信息

Brenner A J, Harris E D

机构信息

Department of Biochemistry and Biophysics, Texas A&M University, College Station 77843, USA.

出版信息

Anal Biochem. 1995 Mar 20;226(1):80-4. doi: 10.1006/abio.1995.1194.

DOI:10.1006/abio.1995.1194

PMID:7785783

Abstract

We describe a direct colorimetric assay for copper in serum and biological samples using 2,2'-bicinchoninic acid (BCA), a common reagent in most laboratories. BCA offers the advantage of being highly sensitive and specific for Cu(I) which rapidly forms an intense purple complex in the presence of BCA. The complex has peak absorbances at 562 and 354.5 nm with molar absorptivities of 7.7 x 10(3) liter mol-1 cm-1 and 4.6 x 10(4) liter mol-1 cm-1, respectively. Interference by other metal ions, pH, and detergents is minimal and the results correlate strongly with atomic absorption spectrophotometry.

摘要

我们描述了一种使用2,2'-二喹啉甲酸（BCA）对血清和生物样品中的铜进行直接比色测定的方法，BCA是大多数实验室常用的试剂。BCA对Cu(I)具有高灵敏度和特异性，在BCA存在下，Cu(I)会迅速形成强烈的紫色络合物。该络合物在562和354.5 nm处有吸收峰，摩尔吸光系数分别为7.7×10³升·摩尔⁻¹·厘米⁻¹和4.6×10⁴升·摩尔⁻¹·厘米⁻¹。其他金属离子、pH值和去污剂的干扰极小，并且结果与原子吸收分光光度法密切相关。