Marshall T, Williams K M
Biochemistry Research Laboratory, School of Pharmaceutical & Chemical Sciences, Sunderland Polytechnic, Great Britain.
Anal Biochem. 1991 Nov 1;198(2):352-4. doi: 10.1016/0003-2697(91)90438-y.
The interference of a range of drugs and related substances has been investigated in the Bradford Coomassie brilliant blue (CBB) protein dye-binding assay and the 2,2'-bicinchoninic acid (BCA) protein assays. Chlorpromazine was the only substance to interfere in the CBB assay but the interference was slight. In contrast, the BCA reagent interacted strongly with chlorpromazine, the penicillins, vitamin C, and paracetamol and the mode of interference varied with the test substance. The chlorpromazine produced turbidity and an atypical color. The penicillins show a slow but normal color response while vitamin C and paracetamol gave an immediate and intense response.
在考马斯亮蓝（CBB）蛋白质染料结合测定法和二喹啉甲酸（BCA）蛋白质测定法中，研究了一系列药物及相关物质的干扰情况。氯丙嗪是唯一对CBB测定有干扰的物质，但干扰程度较轻。相比之下，BCA试剂与氯丙嗪、青霉素、维生素C和对乙酰氨基酚有强烈相互作用，且干扰模式因测试物质而异。氯丙嗪产生浑浊和非典型颜色。青霉素呈现缓慢但正常的颜色反应，而维生素C和对乙酰氨基酚则产生即时且强烈的反应。
