Huang Hui-huang, Wang Si-yu, Wang Hui-fen, Fu Jun-liang, Han Ping, Wang Fu-sheng
Academy of Military Medical Science, Beijing 100850, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Jun;26(6):536-8.
To investigate phenotypical and functional characteristics of the CD39(+);FoxP3(+);regulatory T (Treg) cells in humans.
We collected peripheral blood from 10 healthy volunteers and separated PBMC by using density gradient centrifugation, and then detected the expression of CTLA-4, HLA-DR, CD45RO and Ki-67 on CD39(+);FoxP3(+); and CD39(-);FoxP3(+);Treg cells by using flow cytometric analysis. [(3);H]-thymidine incorporation assay and ELISA were used to monitor the suppressive capacity of CD39(+); FoxP3(+); Treg cells on proliferation and IFN-gamma secretion of Treg-depleted PBMC.
The expression of CTLA-4, HLA-DR, CD45RO and Ki-67 was significantly higher in CD39(+);FoxP3(+); Treg cells than those in CD39(-);FoxP3(+); Treg cells. The suppressive capacity of CD39(+);FoxP3(+); Treg cells on proliferation and IFN-gamma secretion of Treg-depleted PBMC was significantly higher than those of FoxP3(+);CD39(-);Treg cells.
CD39(+);Fxop3(+);Treg subset may play an essential role in immune regulation of Treg, and CD39 can be used as a surface marker to identify the functional Treg cells.
研究人类CD39(+)；FoxP3(+)；调节性T（Treg）细胞的表型和功能特征。
采集10名健康志愿者的外周血，采用密度梯度离心法分离外周血单个核细胞（PBMC），然后通过流式细胞术分析检测CD39(+)；FoxP3(+)；以及CD39(-)；FoxP3(+)；Treg细胞上CTLA-4、HLA-DR、CD45RO和Ki-67的表达。采用[3H]-胸腺嘧啶核苷掺入试验和酶联免疫吸附测定（ELISA）监测CD39(+)；FoxP3(+)；Treg细胞对去除Treg的PBMC增殖和γ干扰素分泌的抑制能力。
CD39(+)；FoxP3(+)；Treg细胞中CTLA-4、HLA-DR、CD45RO和Ki-67的表达显著高于CD39(-)；FoxP3(+)；Treg细胞。CD39(+)；FoxP3(+)；Treg细胞对去除Treg的PBMC增殖和γ干扰素分泌的抑制能力显著高于FoxP3(+)；CD39(-)；Treg细胞。
CD39(+)；Fxop3(+)；Treg亚群可能在Treg的免疫调节中起重要作用，且CD39可作为识别功能性Treg细胞的表面标志物。
