Prinz H, Jürss R, Maelicke A
Max-Planck-Institut, Rheinlanddamm 201, 4600 Dortmund, West-Germany.
Neurochem Int. 1980;2C:251-6. doi: 10.1016/0197-0186(80)90032-7.
We have studied the interaction of NBD-n-acylcholines, fluorescent analogs of acetylcholine with agonist action, with acetylcholine receptor and acetylcholine esterase (EC 3.1.1.7) from Electrophorus electricus. The rates of association of the fluorescent ligands with the two proteins are closely similar during the initial phase of binding, the second order rate constants being of the order of 2 x 10(8) M(?1) s(?1) at physiological salt conditions.
我们研究了NBD - N - 酰基胆碱（乙酰胆碱的荧光类似物，具有激动剂作用）与电鳗乙酰胆碱受体及乙酰胆碱酯酶（EC 3.1.1.7）之间的相互作用。在结合的初始阶段，荧光配体与这两种蛋白质的结合速率非常相似，在生理盐条件下，二级速率常数约为2×10⁸ M⁻¹ s⁻¹。
