Legay C, Faudon M, Ternaux J P
INSERM U.6 and GR 45 CNRS, 280 Bd Sainte-Marguerite, 13009 Marseille, France.
Neurochem Int. 1983;5(3):275-84. doi: 10.1016/0197-0186(83)90029-3.
A biochemical study of the endogenous levels of serotonin (5-HT), noradrenaline (NA) and the activity of choline acetyltransferase (CAT) was carried out in the intestinal tract of the rat. High levels of 5-HT and NA were detected in the caecum and the colon. These anatomical regions also presented the highest activity of CAT. Similar activities of CAT were detected, after dissection, in the mucosa and the muscular layers containing the enteric plexuses. During the day-night cycle, 5-HT and NA amounts showed significant variations as a function of time. Treatment with pargyline (75 mg kg(?1)), a monoamine oxidase inhibitor, resulted in an increase in 5-HT content with parallel modifications in CAT activity. In spite of an important decrease in 5-HT endogenous level in the caecum of rats pretreated with parachlorophenylalanine (300 mg kg(?1)), no significant change in CAT activity was detected whatever was the duration of the treatment. ?-Methylparatyrosine (100 mg kg(?1)), known to block the synthesis of NA, did not affect the CAT activity in the caecum.
对大鼠肠道内血清素（5-羟色胺，5-HT）、去甲肾上腺素（NA）的内源性水平以及胆碱乙酰转移酶（CAT）的活性进行了生化研究。在盲肠和结肠中检测到高水平的5-HT和NA。这些解剖区域的CAT活性也最高。解剖后，在含有肠神经丛的黏膜和肌层中检测到相似的CAT活性。在昼夜周期中，5-HT和NA的量随时间呈现显著变化。用单胺氧化酶抑制剂优降宁（75毫克/千克）治疗，导致5-HT含量增加，同时CAT活性发生相应改变。尽管用对氯苯丙氨酸（300毫克/千克）预处理的大鼠盲肠中5-HT内源性水平显著降低，但无论治疗持续时间如何，均未检测到CAT活性有显著变化。已知能阻断NA合成的α-甲基对酪氨酸（100毫克/千克），对盲肠中的CAT活性没有影响。
