Legay C, Faudon M, Héry F, Ternaux J P
INSERM-U.6 and GR.45 CNRS, 280 Bd Sainte-Marguerite, 13009 Marseille, France.
Neurochem Int. 1983;5(5):571-7. doi: 10.1016/0197-0186(83)90048-7.
The presence of serotonin (5-HT) in dissected intestinal muscular wall of the caecum was demonstrated by the determination of endogenous level of the amine by both spectrofluorimetric and radioenzymatic assays. Biosynthesis of [(3)H]5-HT from [(3)H]tryptophan in in vitro conditions revealed the presence of tryptophan hydroxylase in these muscular layers and therefore strongly suggest the presence of serotoninergic neurons. Following dissection of the mucosa from the muscular layers containing the nerve plexuses, endogenous 5-HT and 5-hydroxyindol acetic acid levels as well as amounts of [(3)H]5-HT synthesized from [(3)H]tryptophan were always higher than those found in intact fragments of the caecum. These results are discussed in terms of metabolic processes involved in the regulation between the two 5-HT containing compartments.
通过荧光分光光度法和放射酶分析法测定胺的内源性水平，证实了盲肠解剖后的肠肌壁中存在血清素（5-羟色胺，5-HT）。在体外条件下由[³H]色氨酸生物合成[³H]5-HT，揭示了这些肌层中存在色氨酸羟化酶，因此有力地表明存在5-羟色胺能神经元。从含有神经丛的肌层剥离黏膜后，内源性5-HT和5-羟吲哚乙酸水平以及由[³H]色氨酸合成的[³H]5-HT量总是高于在完整盲肠片段中发现的水平。根据参与两个含5-HT区室之间调节的代谢过程对这些结果进行了讨论。
