Kintampo Health Research Centre, Ghana.
Vaccine. 2010 Jul 5;28(30):4738-41. doi: 10.1016/j.vaccine.2010.05.001. Epub 2010 May 18.
Vaccines are usually assessed by analyses of their safety and immunogenicity to determine the effectiveness of eliciting antibody responses against target organisms. However, it is equally important to establish antibody affinity because of its specific role in protection from infection. Antibody affinity can be determined by comparisons of various antibody concentrations in dose-response curves. During a study on the immunogenicity of a pentavalent vaccine in 888 infants, antibody affinity analyses of the hepatitis B and Haemophilus influenzae type b components were investigated in infants given 15mg RE vitamin A with their vaccination and those who were not given vitamin A. In this paper we present the results of 222 infants; a 25% sub-sample of the original study. Analyses were carried out using dilutions of serum samples from fitted values corresponding to optical densities from antibody detection assays. These were obtained from the ligand binding equation and mid point titres in dose-response curves were then calculated. Vitamin A supplementation had no effect on the midpoint titres of Hepatitis B and H. influenzae type b vaccine derived antibodies. The significant effect of vitamin A supplementation on the Hepatitis B vaccine component observed in a previous seroprotection analysis is probably due to the amount of antibodies since affinity was unaffected.
疫苗通常通过分析其安全性和免疫原性来评估，以确定针对目标生物体产生抗体反应的有效性。然而，确定抗体亲和力也同样重要，因为它在防止感染方面具有特定作用。可以通过比较剂量反应曲线中各种抗体浓度来确定抗体亲和力。在对 888 名婴儿进行五价疫苗免疫原性研究期间，对接受和未接受 15mg RE 维生素 A 疫苗接种的婴儿的乙型肝炎和流感嗜血杆菌 b 型成分的抗体亲和力进行了分析。本文介绍了对 222 名婴儿的研究结果；这是原始研究的 25%子样本。分析是使用与抗体检测分析的光密度相对应的拟合值的血清样本稀释液进行的。这些是从配体结合方程中获得的，然后计算出剂量反应曲线中的中点滴度。维生素 A 补充剂对乙型肝炎和流感嗜血杆菌 b 型疫苗衍生抗体的中点滴度没有影响。在之前的血清保护分析中观察到维生素 A 补充剂对乙型肝炎疫苗成分的显著影响，可能是由于抗体数量的影响，因为亲和力没有受到影响。
