Department of Biology, Rider University, Lawrenceville, NJ 08648-3099, USA.
Immunobiology. 2011 Jan-Feb;216(1-2):256-64. doi: 10.1016/j.imbio.2010.04.002. Epub 2010 Apr 10.
The T cell composition of the peritoneal cavity (PerC) in naïve BALB/c, C57BL/6, DBA/2J, and B-1 B cell-defective BALB.xid mice was investigated. The BALB.xid PerC T cell pool had a high CD4:CD8 T cell ratio relative to the other strains whose ratios were similar to those found in their lymph node and spleen. All mice had significant representation of T cells with an activated (CD25(+), GITR(hi), CD44(hi), CD45RB(lo), CD62L(lo)) phenotype and low numbers of Foxp3(+) T(reg) cells in their PerC. Despite a phenotype indicative of activation, peritoneal T cell responses to CD3 ligation were very low for C57BL/6 and BALB.xid, but not BALB/c, mice. Enzyme inhibition and cytokine neutralization studies revealed active suppression of the T cell response mediated by the macrophages that represent a significant portion of PerC leucocytes. Driven by IFNγ to express iNOS, macrophages suppressed T cell activation in vitro by arginine catabolism. Although BALB/c T cells were also in a macrophage-dense environment their limited IFNγ production failed to trigger suppression. This difference between BALB/c and BALB.xid PerC T cells suggests a role for xid in shaping macrophage-mediated immune regulation.
研究了 naive BALB/c、C57BL/6、DBA/2J 和 B-1 B 细胞缺陷型 BALB.xid 小鼠腹腔（PerC）中的 T 细胞组成。与其他品系相比，BALB.xid PerC T 细胞池具有较高的 CD4：CD8 T 细胞比例，而其他品系的比例与它们在淋巴结和脾脏中的比例相似。所有小鼠的 PerC 中都有大量表达激活表型（CD25(+)、GITR(hi)、CD44(hi)、CD45RB(lo)、CD62L(lo))的 T 细胞和少量 Foxp3(+)T(reg)细胞。尽管 PerC T 细胞表现出激活表型，但 C57BL/6 和 BALB.xid 小鼠对 CD3 结合的腹腔 T 细胞反应非常低，但 BALB/c 小鼠则不然。酶抑制和细胞因子中和研究表明，巨噬细胞介导的 T 细胞反应受到抑制，巨噬细胞在 PerC 白细胞中占很大一部分。巨噬细胞在 IFNγ 的驱动下表达 iNOS，通过精氨酸代谢抑制 T 细胞活化。尽管 BALB/c T 细胞也处于富含巨噬细胞的环境中，但它们有限的 IFNγ 产生未能引发抑制。BALB/c 和 BALB.xid PerC T 细胞之间的这种差异表明 xid 在塑造巨噬细胞介导的免疫调节中起作用。
