Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Am J Hypertens. 2010 Sep;23(9):960-6. doi: 10.1038/ajh.2010.104. Epub 2010 May 20.
Vascular endothelial growth factor (VEGF) is important in atherosclerotic development. We examined two VEGF polymorphisms, including +405 C/G (rs2010963) and -2578C/A (rs699947), to assess their relation to the extent of coronary atherosclerosis.
We recruited 398 patients with advanced coronary artery disease (CAD). Angiographic "clinical vessel score" (CVS, 1-3 vessels) and "diffuse score" (DS, 0-11.5) were used to evaluate the extent of coronary atherosclerosis. CVS are further categorized into single- (SVD) and multi-vessel disease (MVD: 2 and 3 vessels). Genotyping was performed by TaqMan assay.
The frequency of +405 C/G genotype was correlated with CVS and DS (P = 0.016 and 0.016, respectively). The +405C allele frequency was significantly increased in patients with MVD (P = 0.020). The odds ratio (OR) for the CC to the GG genotype was 2.92 (95% confidence interval (CI): 1.31-6.55, P = 0.007). Univariate and logistic regression analysis showed a significant independent association of +405 C/G genotype with SVD/MVD (P = 0.010 and 0.015, respectively) in the recessive model. Furthermore, there is a synergistic effect of CC genotype and diabetes on the occurrence of MVD (P = 0.001 for interaction). There is also a significant independent association of +405 C/G genotype in the recessive model with DS in the uni- and multivariate regression analysis (P = 0.013 and 0.005, respectively). No association was found between VEGF -2578C/A polymorphism and both atherosclerotic parameters.
The VEGF +405C/G polymorphism is a novel genetic factor to influence the extent of coronary atherosclerosis in the Chinese CAD population.
血管内皮生长因子（VEGF）在动脉粥样硬化的发展中很重要。我们研究了两种 VEGF 多态性，包括 +405 C/G（rs2010963）和 -2578C/A（rs699947），以评估它们与冠状动脉粥样硬化程度的关系。
我们招募了 398 名患有晚期冠状动脉疾病（CAD）的患者。血管造影的“临床血管评分”（CVS，1-3 支血管）和“弥漫评分”（DS，0-11.5）用于评估冠状动脉粥样硬化的程度。CVS 进一步分为单支血管疾病（SVD）和多支血管疾病（MVD：2 支和 3 支血管）。采用 TaqMan 法进行基因分型。
+405 C/G 基因型的频率与 CVS 和 DS 相关（P=0.016 和 0.016）。MVD 患者的+405C 等位基因频率明显升高（P=0.020）。CC 基因型到 GG 基因型的优势比（OR）为 2.92（95%置信区间（CI）：1.31-6.55，P=0.007）。单变量和逻辑回归分析显示，在隐性模型中，+405 C/G 基因型与 SVD/MVD 具有显著的独立关联（P=0.010 和 0.015）。此外，CC 基因型和糖尿病对 MVD 的发生有协同作用（交互作用 P=0.001）。在单变量和多变量回归分析的隐性模型中，+405 C/G 基因型与 DS 也存在显著的独立关联（P=0.013 和 0.005）。VEGF-2578C/A 多态性与两种动脉粥样硬化参数均无关。
VEGF +405C/G 多态性是影响中国 CAD 人群冠状动脉粥样硬化程度的一个新的遗传因素。
