Ansari M N, Nigam G K, Bhandari Uma
Department of Pharmacology, Faculty of Pharmacy, Jamia Hamdard (Hamdard University), New Delhi-110 062, India.
Indian J Pharm Sci. 2009 May;71(3):270-5. doi: 10.4103/0250-474X.56025.
The present study was designed to investigate the effect of folic acid on homocysteine, lipid profile and hematological changes in methionine-induced hyperhomocysteinemic rats. Hyperhomocysteinemia was induced by methionine (1 g/kg, p.o.) administration for 30 days. Biochemical and hematological observations were further substantiated with histopathological examination. The increase in homocysteine, total cholesterol, low density lipoprotein-cholesterol, very low density lipoprotein-cholesterol and triglycerides levels with reduction in the levels of high density lipoprotein in serum were the salient features observed in methionine treated toxicologic control rats (i.e. group II). Hematological observations of the peripheral blood smears of toxicologic rats also showed crenation of red blood cells membrane and significant (P<0.01) increase in total leukocyte count, differential leukocyte count and platelet counts with significant (P<0.01) decrease in the mean hemoglobin levels, as compared to vehicle control rats. Administration of folic acid (100 mg/kg, p.o.) for 30 days to methionine- induced hyperhomocysteinemic rats produced a significant (P< 0.01) decrease in the levels of homocysteine, total cholesterol, low density lipoprotein-cholesterol, very low density lipoprotein-cholesterol and triglycerides with significant (P< 0.01) increase in high density lipoprotein-cholesterol levels in serum when compared with toxicologic control rats. The present study, for the first time, investigates the effect of folic acid treatment on hematological changes in rats with methionine-induced hyperhomocysteinemia.
本研究旨在探讨叶酸对蛋氨酸诱导的高同型半胱氨酸血症大鼠的同型半胱氨酸、血脂谱及血液学变化的影响。通过口服给予蛋氨酸（1 g/kg）30天诱导高同型半胱氨酸血症。生化和血液学观察结果通过组织病理学检查进一步得到证实。在经蛋氨酸处理的毒理学对照大鼠（即第二组）中观察到的显著特征包括血清中同型半胱氨酸、总胆固醇、低密度脂蛋白胆固醇、极低密度脂蛋白胆固醇和甘油三酯水平升高，同时高密度脂蛋白水平降低。与溶剂对照大鼠相比，毒理学大鼠外周血涂片的血液学观察还显示红细胞膜出现皱缩，总白细胞计数、分类白细胞计数和血小板计数显著（P<0.01）增加，而平均血红蛋白水平显著（P<0.01）降低。对蛋氨酸诱导的高同型半胱氨酸血症大鼠口服叶酸（100 mg/kg）30天，与毒理学对照大鼠相比，血清中同型半胱氨酸、总胆固醇、低密度脂蛋白胆固醇、极低密度脂蛋白胆固醇和甘油三酯水平显著（P<0.01）降低，高密度脂蛋白胆固醇水平显著（P<0.01）升高。本研究首次探讨了叶酸治疗对蛋氨酸诱导的高同型半胱氨酸血症大鼠血液学变化的影响。
