Department of Pharmacology, Faculty of Pharmacy, Hamdard University, New Delhi, India.
Indian J Pharmacol. 2008 Aug;40(4):152-7. doi: 10.4103/0253-7613.43161.
The present study was designed to evaluate the effect of the aqueous extract of Embelia ribes Burm fruits on methionine-induced hyperhomocysteinemia, hyperlipidemia and oxidative stress in albino rats.
Adult male Wistar albino rats were fed with the aqueous extract of Embelia ribes (100 and 200 mg/kg, p.o.) for 30 days. Hyperhomocysteinemia was induced by methionine treatment (1 g/kg, p.o.) for 30 days and folic acid (100 mg/kg, p.o.) was used as a standard drug. The animals were evaluated for various biochemical parameters in serum and brain homogenates, followed by histopathological studies at the end of the study.
Administration of methionine (1 g/kg, p.o.) for 30 days to vehicle control rats produced significant increase (P < 0.01) in homocysteine, lactate dehydrogenase (LDH), total cholesterol, triglycerides, low density lipoprotein (LDL-C), very low density lipoprotein (VLDL-C) levels in serum and lipid peroxides (LPO) levels in brain homogenates, with reduction in high density lipoprotein (HDL-C) levels in serum, and glutathione (GSH) content in brain homogenates, as compared to vehicle control rats. Administration of the aqueous extract of Embelia ribes (100 and 200 mg/kg, p.o.) for 30 days, to hyperhomocysteinemic rats, significantly (P < 0.01) decreased the levels of homocysteine, LDH, total cholesterol, triglycerides, LDL-C and VLDL-C and increased the HDL-C levels in serum. In addition, a significant (P < 0.01) decrease in LPO levels with increase in GSH content was observed in hyperhomocysteinemic rats treated with the aqueous extract of Embelia ribes. The results were comparable to those obtained with folic acid, a standard antihyperhomocysteinemic drug.
The present results provide clear evidence that the aqueous extract of Embelia ribes treatment enhances the antioxidant defense against methionine-induced hyperhomocysteinemia, hyperlipidemia and oxidative stress in brain.
本研究旨在评估余甘子水提物对蛋氨酸诱导的高同型半胱氨酸血症、高血脂症和氧化应激的影响。
成年雄性 Wistar 白化大鼠连续 30 天给予余甘子水提物（100 和 200 mg/kg，po）。通过给予蛋氨酸（1 g/kg，po）30 天诱导高同型半胱氨酸血症，并使用叶酸（100 mg/kg，po）作为标准药物。在研究结束时，评估动物血清和脑组织匀浆中的各种生化参数，并进行组织病理学研究。
给予蛋氨酸（1 g/kg，po）30 天，可使载体对照组大鼠的同型半胱氨酸、乳酸脱氢酶（LDH）、总胆固醇、甘油三酯、低密度脂蛋白（LDL-C）、极低密度脂蛋白（VLDL-C）水平显著升高（P<0.01），血清高密度脂蛋白（HDL-C）水平降低，脑组织匀浆脂质过氧化物（LPO）水平升高。与载体对照组大鼠相比，给予余甘子水提物（100 和 200 mg/kg，po）30 天，可显著降低高同型半胱氨酸血症大鼠的同型半胱氨酸、LDH、总胆固醇、甘油三酯、LDL-C 和 VLDL-C 水平，升高血清 HDL-C 水平。此外，余甘子水提物还可显著降低高同型半胱氨酸血症大鼠的 LPO 水平，增加 GSH 含量（P<0.01）。这些结果与标准抗高同型半胱氨酸血症药物叶酸相似。
本研究结果提供了明确的证据，表明余甘子水提物治疗可增强抗氧化防御系统，减轻蛋氨酸诱导的高同型半胱氨酸血症、高血脂症和氧化应激对大脑的影响。
