School of Biological Sciences, The University of Queensland, St. Lucia, QLD, 4072, Australia.
Plant Cell Rep. 2010 Aug;29(8):835-44. doi: 10.1007/s00299-010-0868-y. Epub 2010 May 21.
Crop adaptation to abiotic stresses requires alterations in expression of a large number of stress protection genes and their regulators, including transcription factors. In this study, the expression levels of ten MYB transcription factor genes from wheat (Triticum aestivum) were examined in two recombinant inbred lines contrasting in their salt tolerance in response to salt or drought stress. Quantitative RT-PCR analysis revealed that four MYB genes were consistently up-regulated in the seedling roots of both genotypes under short-term salt treatment. Three MYB genes were found to be up-regulated in both genotypes under long-term salt stress. One MYB gene was up-regulated in both genotypes under both short- and long-term salt stress. Of these salt up-regulated MYB genes, one MYB gene (TaMYBsdu1) was markedly up-regulated in the leaf and root of wheat under long-term drought stress. In addition, TaMYBsdu1 showed higher expression levels in the salt-tolerant genotype than in the susceptible genotype under salt stress. These data suggest that TaMYBsdu1 is a potentially important regulator involved in wheat adaptation to both salt and drought stresses.
作物适应非生物胁迫需要改变大量应激保护基因及其调控因子的表达，包括转录因子。在这项研究中，研究人员检测了两个在盐耐受性方面存在差异的重组自交系中 10 个小麦（Triticum aestivum）MYB 转录因子基因的表达水平，以响应盐或干旱胁迫。定量 RT-PCR 分析显示，在短期盐处理下，两个基因型的幼苗根中均有 4 个 MYB 基因持续上调。在长期盐胁迫下，发现 3 个 MYB 基因在两个基因型中均上调。在短期和长期盐胁迫下，有一个 MYB 基因在两个基因型中均上调。在这些盐诱导的 MYB 基因中，一个 MYB 基因（TaMYBsdu1）在长期干旱胁迫下，在小麦的叶片和根中显著上调。此外，在盐胁迫下，TaMYBsdu1 在耐盐基因型中的表达水平高于易感基因型。这些数据表明，TaMYBsdu1 是一个潜在的重要调节因子，参与小麦对盐胁迫和干旱胁迫的适应。
