Luo Guijie, Cai Weijia, Wang Hao, Liu Wei, Liu Xu, Shi Shizheng, Wang Lei
Suqian Institute of Agricultural Sciences, Jiangsu Academy of Agricultural Sciences, Suqian 223800, China.
Jiangsu Academy of Forestry, Nanjing 211153, China.
Plants (Basel). 2024 Aug 15;13(16):2264. doi: 10.3390/plants13162264.
is a Sieb.et tree of the family Scrophulariaceae. It has become an important short-to-medium-term fast-growing multi-purpose tree species in China due to its rapid growth, strong adaptability, and excellent material properties. MYB transcription factors in plants have numerous and diverse functions, playing important roles in various aspects such as plant stress response. To investigate the function of MYB transcription factors in , this study used PCR technology to clone the gene from . The homology of PfMYB44 and SiMYB44 () was the highest. Expression analysis results showed that was expressed in the root, stem, young leaf, and mature leaf of , with the highest content in the root. Cold, drought, hot, salt, and ABA treatments could increase the expression level of . Overexpression- plants were constructed, and physiological and molecular analysis showed that could positively regulate salt and drought stresses. Under drought stress, the expression levels of , , and in transgenic lines were significantly induced. Salt stress induced the expression of , , and genes, and the relative expression levels of these genes in transgenic were higher. In conclusion, the functional study of laid a certain foundation for the study of stress resistance, and was helpful to the study of its stress resistance mechanism and the cultivation of new stress resistance varieties.
是玄参科的一种树种。由于其生长迅速、适应性强和优良的材质特性，它已成为中国重要的中短期速生多用途树种。植物中的MYB转录因子具有众多且多样的功能，在植物应激反应等各个方面发挥着重要作用。为了研究MYB转录因子在中的功能，本研究利用PCR技术从克隆了基因。PfMYB44和SiMYB44（）的同源性最高。表达分析结果表明，在的根、茎、幼叶和成熟叶中均有表达，根中的含量最高。冷、旱、热、盐和ABA处理均可提高的表达水平。构建了过表达植株，生理和分子分析表明，可正向调节盐胁迫和干旱胁迫。在干旱胁迫下，转基因株系中的、、和的表达水平显著诱导。盐胁迫诱导了、、和基因的表达，这些基因在转基因中的相对表达水平更高。总之，的功能研究为抗逆性研究奠定了一定基础，有助于其抗逆机制研究和新抗逆品种培育。
