College of Life Science, Jilin Agricultural University, Changchun, 130118, Jilin, China.
Jilin Engineering Research Center Ginseng Genetic Resources Development and Utilization, Changchun, 130118, Jilin, China.
BMC Plant Biol. 2022 Oct 8;22(1):479. doi: 10.1186/s12870-022-03871-8.
As the king of all herbs, the medicinal value of ginseng is self-evident. The perennial nature of ginseng causes its quality to be influenced by various factors, one of which is the soil environment. During plant growth and development, MYB transcription factors play an important role in responding to abiotic stresses and regulating the synthesis of secondary metabolites. However, there are relatively few reports on the MYB transcription factor family in Panax ginseng.
This study identified 420 PgMYB transcripts under 117 genes ID in the Jilin ginseng transcriptome database. Phylogenetic analysis showed that PgMYB transcripts in Jilin ginseng were classified into 19 functional subclasses. The GO annotation result indicated that the functional differentiation of PgMYB transcripts was annotated to 11 functional nodes at GO Level 2 in ginseng. Expression pattern analysis of PgMYB transcripts based on the expression data (TPM) that PgMYB transcripts were revealed spatiotemporally specific in expression patterns. We performed a weighted network co-expression network analysis on the expression of PgMYB transcripts from different samples. The co-expression network containing 51 PgMYB transcripts was formed under a soft threshold of 0.85, revealing the reciprocal relationship of PgMYB in ginseng. Treatment of adventitious roots of ginseng with different concentrations of NaCl revealed four up-regulated expression of PgMYB transcripts that can candidate genes for salt resistance studies in ginseng.
The present findings provide data resources for the subsequent study of the functions of MYB transcription factor family members in ginseng, and provide an experimental basis for the anti-salt functions of MYB transcription factors in Panax ginseng.
作为百草之王，人参的药用价值不言而喻。人参的多年生特性导致其质量受到多种因素的影响，其中之一是土壤环境。在植物生长发育过程中，MYB 转录因子在响应非生物胁迫和调节次生代谢物合成方面发挥着重要作用。然而，关于人参 MYB 转录因子家族的报道相对较少。
本研究在吉林人参转录组数据库中，根据 117 个基因 ID 鉴定出 420 个 PgMYB 转录本。系统发育分析表明，吉林人参 PgMYB 转录本分为 19 个功能亚类。GO 注释结果表明，PgMYB 转录本的功能分化在人参中被注释到 GO 水平 2 的 11 个功能节点。基于表达数据（TPM）的 PgMYB 转录本表达模式分析表明，PgMYB 转录本在表达模式上具有时空特异性。我们对来自不同样本的 PgMYB 转录本的表达进行了加权网络共表达网络分析。在软阈值为 0.85 的条件下，形成了包含 51 个 PgMYB 转录本的共表达网络，揭示了人参中 PgMYB 转录本的相互关系。用不同浓度的 NaCl 处理人参不定根，发现 4 个上调表达的 PgMYB 转录本，可作为人参耐盐研究的候选基因。
本研究结果为进一步研究人参 MYB 转录因子家族成员的功能提供了数据资源，为研究人参 MYB 转录因子的抗盐功能提供了实验依据。
