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苹果片中硫酸酸化钠盐的抗褐变和抗菌性能。

Antibrowning and antimicrobial properties of sodium acid sulfate in apple slices.

机构信息

U.S. Dept. of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA.

出版信息

J Food Sci. 2009 Nov-Dec;74(9):M485-92. doi: 10.1111/j.1750-3841.2009.01362.x.

Abstract

There are few available compounds that can both control browning and enhance microbial safety of fresh-cut fruits. In the present study, the antibrowning ability of sodium acid sulfate (SAS) on "Granny Smith" apple slices was first investigated in terms of optimum concentration and treatment time. In a separate experiment, the apple slices were treated with water or 3% of SAS, calcium ascorbate, citric acid, or acidified calcium sulfate for 5 min. Total plate count, color, firmness, and tissue damage were assessed during a 21-d storage at 4 degrees C. Results showed that the efficacy of SAS in inhibiting browning of apple slices increased with increasing concentration. A minimum 3% of SAS was needed to achieve 14 d of shelf life. Firmness was not significantly affected by SAS at 3% or lower concentrations. Antibrowning potential of SAS was similar for all treatment times ranging from 2 to 10 min. However, SAS caused some skin discoloration of apple slices. When cut surface of apple slices were stained with a fluorescein diacetate solution, tissue damage could be observed under a microscope even though visual damage was not evident. Among the antibrowning agents tested, SAS was the most effective in inhibiting browning and microbial growth for the first 14 d. Total plate count of samples treated with 3% SAS was significantly lower than those treated with calcium ascorbate, a commonly used antibrowning agent. Our results suggested that it is possible to use SAS to control browning while inhibiting the growth of microorganisms on the apple slices if the skin damage can be minimized. Practical Application: Fresh-cut apples have emerged as one of the popular products in restaurants, schools, and food service establishments as more consumers demand fresh, convenient, and nutritious foods. Processing of fresh-cut apples induces mechanical damage to the fruit and exposes apple tissue to air, resulting in the development of undesirable tissue browning. The fresh-cut industry currently uses antibrowning agents to prevent discoloration. However, the antibrowning solutions can become contaminated with human pathogens such as Listeria monocytogenes, and washing of apple slices with the contaminated solutions can result in the transfer of pathogens to the product. It would be ideal if an antibrowning compound prevented the proliferation of human pathogens in solutions and minimized the growth of pathogens during storage. The study was conducted to investigate antibrowning and antimicrobial properties of sodium acid sulfate (SAS) in comparison with other common antibrowning agents on Granny Smith apples. Results showed that among the antimicrobial agents we tested, SAS was the most effective in inhibiting browning and microbial growth for 14 d at 4 degrees C. However, SAS caused some skin discoloration of apple slices. Overall, SAS can potentially be used to inhibit tissue browning while reducing the microbial growth on apple slices. The information is useful for the fresh-cut produce industry to enhance microbial safety of fresh-cut apples while minimizing browning, thus increasing the consumption of the health benefiting fresh fruit.

摘要

目前可用于控制褐变并提高鲜切水果微生物安全性的化合物很少。本研究首先考察了亚硫酸氢钠(SAS)对“青苹果”苹果片的最佳浓度和处理时间的抑褐能力。在另一个实验中,将苹果片用 3%的 SAS、抗坏血酸钙、柠檬酸或酸化硫酸钙处理 5 分钟。在 4°C 下储存 21 天期间评估了总平板计数、颜色、硬度和组织损伤。结果表明,SAS 抑制苹果片褐变的效果随浓度的增加而增加。需要至少 3%的 SAS 才能达到 14 天的货架期。在 3%或更低浓度下,SAS 对硬度没有显著影响。SAS 的抑褐效果在 2 至 10 分钟的所有处理时间内相似。然而,SAS 会使苹果片的表皮变色。当用荧光二乙酸溶液染色苹果片的切面时,即使肉眼看不到明显的损伤,在显微镜下也可以观察到组织损伤。在所测试的抑褐剂中,SAS 在最初 14 天内对抑制褐变和微生物生长最有效。用 3%的 SAS 处理的样品的总平板计数明显低于用常用的抑褐剂抗坏血酸钙处理的样品。我们的结果表明,如果可以将表皮损伤最小化,那么使用 SAS 控制褐变的同时也可以抑制苹果片上微生物的生长。

实际应用

鲜切苹果已成为餐馆、学校和食品服务机构中最受欢迎的产品之一,因为越来越多的消费者要求食用新鲜、方便和营养丰富的食品。鲜切苹果的加工会对水果造成机械损伤,并使苹果组织暴露在空气中,导致不希望出现的组织褐变。鲜切行业目前使用抑褐剂来防止变色。然而,抑褐溶液可能会受到李斯特菌等人类病原体的污染,用受污染的溶液清洗苹果片会导致病原体转移到产品上。如果有一种抑褐化合物可以防止溶液中人类病原体的增殖,并在储存过程中最大限度地减少病原体的生长,那就太理想了。本研究旨在比较亚硫酸氢钠(SAS)与其他常见抑褐剂对青苹果的抑褐和抗菌特性。结果表明,在所测试的抗菌剂中,SAS 在 4°C 下抑制褐变和微生物生长的效果在 14 天内最为有效。然而,SAS 会使苹果片的表皮变色。总体而言,SAS 可能具有潜在用途,可以抑制苹果片的组织褐变,同时减少苹果片上微生物的生长。这些信息对鲜切农产品行业很有帮助,可以在最小化褐变的同时提高鲜切苹果的微生物安全性,从而增加健康有益的新鲜水果的消费。

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