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亚氯酸钠和丙酸钙的结合降低了鲜切“青苹果”的酶褐变和微生物种群。

Combination of sodium chlorite and calcium propionate reduces enzymatic browning and microbial population of fresh-cut "Granny Smith" apples.

机构信息

Tianjin Key Lab. of Postharvest Physiology and Storage of Agricultural Products, Natl. Engineering and Technology Research Center for Preservation of Agriculture Products, Tianjin 300384, China.

出版信息

J Food Sci. 2010 Mar;75(2):M72-7. doi: 10.1111/j.1750-3841.2009.01470.x.

Abstract

Tissue browning and microbial growth are the main concerns associated with fresh-cut apples. In this study, effects of sodium chlorite (SC) and calcium propionate (CP), individually and combined, on quality and microbial population of apple slices were investigated. "Granny Smith" apple slices, dipped for 5 min in CP solutions at 0%, 0.5%, 1%, and 2% (w/v) either alone or in combination with 0.05% (w/v) SC, were stored at 3 and 10 degrees C for up to 14 d. Color, firmness, and microflora population were measured at 1, 7, and 14 d of storage. Results showed that CP alone had no significant effect on the browning of cut apples. Even though SC significantly inhibited tissue browning initially, the apple slices turned brown during storage at 10 degrees C. The combination of CP and SC was able to inhibit apple browning during storage. Samples treated with the combination of SC with CP did not show any detectable yeast and mold growth during the entire storage period at 3 degrees C. At 10 degrees C, yeast and mold count increased on apple slices during storage while CP reduced the increase. However, high concentrations of CP reduced the efficacy of SC in inactivating E. coli inoculated on apples. Overall, our results suggested that combination of SC with 0.5% and 1% CP could be used to inhibit tissue browning and maintain firmness while reducing microbial population. Practical Application: Apple slices, which contain antioxidants and other nutrient components, have emerged as popular snacks in food service establishments, school lunch programs, and for family consumption. However, the further growth of the industry is limited by product quality deterioration caused by tissue browning, short shelf-life due to microbial growth, and possible contamination with human pathogens during processing. Therefore, this study was conducted to develop treatments to reduce microbial population and tissue browning of "Granny Smith" apple slices. Results showed that an antimicrobial compound, sodium chlorite, is effective in not only eliminating microbes but also inhibiting tissue browning of apple slices. However, the compound caused tissue softening and its antibrowning effect was short-lived, lasting only for a few days. Combination of the compound with a calcium-containing food additive was able to improve firmness and freshness of apple slices while reducing population of Escherichia coli artificially inoculated on samples and inhibiting the growth of yeast and mold during storage.

摘要

组织褐变和微生物生长是与鲜切苹果相关的主要问题。在这项研究中,单独和组合使用次氯酸钠 (SC) 和丙酸钙 (CP) 对苹果片的质量和微生物种群的影响进行了研究。将“青苹果”苹果片浸泡在 0%、0.5%、1%和 2%(w/v)的 CP 溶液中 5 分钟,单独或与 0.05%(w/v)SC 组合,在 3 和 10°C 下储存长达 14 天。在储存的第 1、7 和 14 天测量颜色、硬度和微生物种群。结果表明,CP 单独使用对切苹果的褐变没有显著影响。尽管 SC 最初能显著抑制组织褐变,但苹果片在 10°C 下储存时会变褐。CP 和 SC 的组合能够抑制苹果的褐变。在整个 3°C 的储存期间,用 SC 与 CP 组合处理的样品未显示出任何可检测到的酵母和霉菌生长。在 10°C 下,随着储存时间的延长,苹果片上的酵母和霉菌数量增加,而 CP 则减少了这种增加。然而,高浓度的 CP 降低了 SC 在苹果上灭活接种的大肠杆菌的效果。总的来说,我们的结果表明,将 SC 与 0.5%和 1%的 CP 组合使用可以抑制组织褐变并保持硬度,同时降低微生物种群。

实际应用

苹果片作为一种富含抗氧化剂和其他营养成分的流行小吃,已在餐饮服务场所、学校午餐计划和家庭消费中崭露头角。然而,由于组织褐变导致产品质量恶化、由于微生物生长导致保质期短以及加工过程中可能受到人类病原体污染,该行业的进一步发展受到限制。因此,进行了这项研究以开发减少“青苹果”苹果片的微生物种群和组织褐变的处理方法。结果表明,一种抗菌化合物次氯酸钠不仅有效消除微生物,而且还能抑制苹果片的组织褐变。然而,该化合物会导致组织软化,其抑制褐变的效果持续时间很短,仅持续几天。将该化合物与含钙的食品添加剂组合使用,可以改善苹果片的硬度和新鲜度,同时减少人工接种在样品上的大肠杆菌的种群,并抑制储存期间酵母和霉菌的生长。

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