Li Yongxin, Wills Ron B H, Golding John B, Huque Roksana
School of Environmental & Life Sciences, University of Newcastle, Ourimbah, NSW 2258, Australia.
J Sci Food Agric. 2015 Mar 30;95(5):945-52. doi: 10.1002/jsfa.6766. Epub 2014 Jun 27.
The postharvest life of fresh-cut apple slices is limited by browning on cut surfaces. Dipping in halide salt solutions was examined for their inhibition of surface browning on 'Granny Smith' apple slices and the effects on biochemical factors associated with browning.
Delay in browning by salts was greatest with chloride = phosphate > sulfate > nitrate with no difference between sodium, potassium and calcium ions. The effectiveness of sodium halides on browning was fluoride > chloride = bromide > iodide = control. Polyphenol oxidase (PPO) activity of tissue extracted from chloride- and fluoride-treated slices was not different to control but when added into the assay solution, NaF > NaCl both showed lower PPO activity at pH 3-5 compared to control buffer. The level of polyphenols in treated slices was NaF > NaCl > control. Addition of chlorogenic acid to slices enhanced browning but NaCl and NaF counteracted this effect. There was no effect of either halide salt on respiration, ethylene production, ion leakage, and antioxidant activity.
Dipping apple slices in NaCl is a low cost treatment with few impediments to commercial use and could be a replacement for other anti-browning additives. The mode of action of NaCl and NaF is through decreasing PPO activity resulting in reduced oxidation of polyphenols.
鲜切苹果片的采后寿命受切割表面褐变的限制。研究了将苹果片浸泡在卤化物盐溶液中对‘澳洲青苹’苹果片表面褐变的抑制作用以及对与褐变相关的生化因素的影响。
盐对褐变的延缓作用以氯化物=磷酸盐>硫酸盐>硝酸盐最为显著,钠、钾和钙离子之间无差异。卤化钠对褐变的有效性为氟化物>氯化物=溴化物>碘化物=对照。从氯化物和氟化物处理的切片中提取的组织的多酚氧化酶(PPO)活性与对照无差异,但当添加到测定溶液中时,在pH 3-5条件下,NaF>NaCl均显示出比对照缓冲液更低的PPO活性。处理切片中的多酚水平为NaF>NaCl>对照。向切片中添加绿原酸会增强褐变,但NaCl和NaF可抵消这种影响。两种卤化物盐对呼吸作用、乙烯生成、离子渗漏和抗氧化活性均无影响。
将苹果片浸泡在NaCl中是一种低成本处理方法,对商业应用几乎没有阻碍,可替代其他抗褐变添加剂。NaCl和NaF的作用方式是通过降低PPO活性,从而减少多酚的氧化。