Dept. of Agricultural Chemistry, NWFP Agricultural Univ., Peshawar, Pakistan.
J Food Sci. 2010 Mar;75(2):M61-4. doi: 10.1111/j.1750-3841.2009.01462.x.
The efficacy of calcium propionate at 2 different doses (0.5% and 1%) against growth and aflatoxins production by Aspergillus flavus (A-2092) was investigated in vitro on Czapek yeast extract agar at different levels of water activity (a(w)) in the range of 0.94 to 0.996a(w). A. flavus spores germinated on all calcium propionate and a(w) treatments; however, 1% calcium propionate at 0.94 a(w) delayed the germination process for up to 10 d. The growing rate of mycelia was slower (0.28 mm/d) at 1% calcium propionate and 0.94 a(w). Aflatoxins (B1, B2, G1, and G2) were also produced minimally (36.1, 1, 1.86, and 1.01 ng/g of media, respectively) at the aforementioned dose rate of calcium propionate and water activity. It was concluded that addition of calcium propionate and a(w) amelioration can prove effective tools for suppressing the germination, growth rate, and aflatoxins production by A. flavus in substrate.
丙酸钙在不同水分活度(a(w))水平下对黄曲霉(A-2092)生长和黄曲霉毒素产生的抑制效果
在体外 Czapek 酵母提取物琼脂上,用 0.94 到 0.996a(w)范围内的两种不同剂量(0.5%和 1%)研究了丙酸钙对黄曲霉生长和黄曲霉毒素产生的抑制效果。黄曲霉孢子在所有丙酸钙和 a(w)处理条件下都能发芽;然而,在 0.94 a(w)的情况下,1%的丙酸钙会将发芽过程延迟最多 10 天。在 1%的丙酸钙和 0.94 a(w)的条件下,菌丝的生长速度较慢(0.28 毫米/天)。在上述丙酸钙剂量率和水分活度下,黄曲霉毒素(B1、B2、G1 和 G2)的产量也最低(分别为 36.1、1、1.86 和 1.01ng/g 培养基)。结论是,添加丙酸钙和改善水分活度可以成为抑制基质中黄曲霉的发芽、生长速度和黄曲霉毒素产生的有效工具。