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从噬菌体fd单链起源处的DNA转录而来的一种RNA，用于向复制形式的转化。

An RNA transcribed from DNA at the origin of phage fd single strand to replicative form conversion.

作者信息

Geider K, Beck E, Schaller H

出版信息

Proc Natl Acad Sci U S A. 1978 Feb;75(2):645-9. doi: 10.1073/pnas.75.2.645.

DOI:10.1073/pnas.75.2.645
PMID:204927
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC411312/
Abstract

Phage fd DNA complexed with DNA binding protein I was used by Escherichia coli RNA polymerase (nucleoside triphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) to synthesize an RNA at the origin of single strand to double strand replication. The isolated ori-RNA gave a simple fingerprint after nucleolytic digestion and has a length of about 30 nucleotides. The characterization of the oligonucleotides from the nuclease digest and the extension of the ori-RNA with DNA polymerase I and subsequent restriction of the DNA gave its exact localization in the fd genome, and its total sequence was deduced from the known DNA sequence in this region.

摘要

与DNA结合蛋白I复合的噬菌体fd DNA被大肠杆菌RNA聚合酶（核苷三磷酸：RNA核苷酸转移酶，EC 2.7.7.6）用于在单链到双链复制起点合成RNA。分离出的ori-RNA经核酸酶消化后给出简单的指纹图谱，长度约为30个核苷酸。对核酸酶消化产生的寡核苷酸进行表征，并通过DNA聚合酶I对ori-RNA进行延伸以及随后对DNA进行限制，确定了其在fd基因组中的精确位置，其完整序列是根据该区域已知的DNA序列推导出来的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e84d/411312/5f34a068ff29/pnas00014-0119-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e84d/411312/b472da4cefda/pnas00014-0118-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e84d/411312/67edfe6102d0/pnas00014-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e84d/411312/5f34a068ff29/pnas00014-0119-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e84d/411312/b472da4cefda/pnas00014-0118-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e84d/411312/67edfe6102d0/pnas00014-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e84d/411312/5f34a068ff29/pnas00014-0119-b.jpg

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本文引用的文献

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A possible role for RNA polymerase in the initiation of M13 DNA synthesis.RNA聚合酶在M13 DNA合成起始过程中的可能作用。
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2826-9. doi: 10.1073/pnas.68.11.2826.
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Conversion of the M13 viral single strand to the double-stranded replicative forms by purified proteins.通过纯化蛋白将M13病毒单链转化为双链复制形式。
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Initiation of deoxyribonucleic acid synthesis. VII. A unique location of the gap in the M13 replicative duplex synthesized in vitro.
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8
Replication of the plasmid pBR322 under the control of a cloned replication origin from the single-stranded DNA phage M13.在来自单链DNA噬菌体M13的克隆复制起点控制下的质粒pBR322的复制。
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4638-42. doi: 10.1073/pnas.77.8.4638.
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脱氧核糖核酸合成的起始。VII. 体外合成的M13复制双链体中缺口的独特位置。
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