白细胞介素-17 在盘状红斑狼疮中的作用。

IL-17 in cutaneous lupus erythematosus.

机构信息

Colentina Clinical Hospital, Bucharest, Romania.

出版信息

Eur J Intern Med. 2010 Jun;21(3):202-7. doi: 10.1016/j.ejim.2010.03.004. Epub 2010 Apr 8.

DOI:10.1016/j.ejim.2010.03.004

PMID:20493423

Abstract

BACKGROUND

Lupus erythematosus (LE) is a heterogeneous disease with broad clinical spectrum from cutaneous to visceral and systemic inflammation. IL-17 isoforms (IL-17A and IL-17F) are proinflammatory cytokines with unclear implications in lupus erythematosus pathogenesis. In this study we focused upon IL-17 in normal and modified lupus skin with a correlative study between local and serological expression.

MATERIAL AND METHODS

89 subjects were recruited and divided in 5 groups-10 patients with psoriasis (disease control group), 13 healthy controls, 26 with discoid chronic lupus (DLE), 23 with systemic lupus erythematosus (SLE) and 17 with subacute lupus erythematosus (SCLE). Blood samples and skin punched-biopsy specimens were performed. Serum IL-17A, IL-17F, and IL-23 concentrations were determined by ELISA. Skin IL-17A and CD4 expression were evaluated by immunohistochemistry.

RESULTS

Immunohistochemical expression of IL-17A was higher in DLE, SCLE and SLE patients than in negative control subjects (all p<0.05). Serum IL-17A concentrations were higher in DLE and SLE patients than in negative controls (p<0.05). Serum IL-17A levels were similar in SCLE and negative controls (p>0.05). Serum IL-17F concentrations were higher in DLE, SCLE and SLE patients than in healthy controls (all p<0.05). In DLE, SCLE, SLE patients and healthy controls we observed comparable levels of IL-23 (p>0.05). Serum anti Ro antibodies correlate with IL-17A+ lymphocytes from SCLE lesion and SLE normal skin (all p<0.05).

CONCLUSION

IL-17 isoforms (IL-17A and IL-17F) are implicated in SLE but also in DLE and SCLE immunopathogenesis.

摘要

背景

红斑狼疮（LE）是一种具有广泛临床表现的异质性疾病，从皮肤到内脏和全身炎症。IL-17 同工型（IL-17A 和 IL-17F）是促炎细胞因子，其在红斑狼疮发病机制中的作用尚不清楚。在这项研究中，我们专注于正常和改良狼疮皮肤中的 IL-17，并进行了局部和血清表达的相关性研究。

材料和方法

招募了 89 名受试者，并分为 5 组-10 名银屑病患者（疾病对照组）、13 名健康对照者、26 名盘状慢性狼疮（DLE）患者、23 名系统性红斑狼疮（SLE）患者和 17 名亚急性红斑狼疮（SCLE）患者。进行了血液样本和皮肤打孔活检。通过 ELISA 测定血清 IL-17A、IL-17F 和 IL-23 浓度。通过免疫组织化学评估皮肤 IL-17A 和 CD4 的表达。

结果

DLE、SCLE 和 SLE 患者的皮肤 IL-17A 免疫组织化学表达高于阴性对照组（均 p<0.05）。DLE 和 SLE 患者的血清 IL-17A 浓度高于阴性对照组（p<0.05）。SLE 患者和阴性对照组的血清 IL-17A 水平相似（p>0.05）。DLE、SCLE 和 SLE 患者的血清 IL-17F 浓度高于健康对照组（均 p<0.05）。在 DLE、SCLE、SLE 患者和健康对照组中，我们观察到 IL-23 的水平相当（p>0.05）。血清抗 Ro 抗体与 SCLE 病变和 SLE 正常皮肤中的 IL-17A+淋巴细胞相关（均 p<0.05）。