Zasadzinski J A, Helm C A, Longo M L, Weisenhorn A L, Gould S A, Hansma P K
Department of Chemical and Nuclear Engineering, University of California, Santa Barbara 93106.
Biophys J. 1991 Mar;59(3):755-60. doi: 10.1016/S0006-3495(91)82288-3.
We present images of the polar or headgroup regions of bilayers of dimyristoyl-phosphatidylethanolamine (DMPE), deposited by Langmuir-Blodgett deposition onto mica substrates at high surface pressures and imaged under water at room temperature with the optical lever atomic force microscope. The lattice structure of DMPE is visualized with sufficient resolution that the location of individual headgroups can be determined. The forces are sufficiently small that the same area can be repeatedly imaged with a minimum of damage. The DMPE molecules in the bilayer appear to have relatively good long-range orientational order, but rather short-range and poor positional order. These results are in good agreement with x-ray measurements of unsupported lipid monolayers on the water surface, and with electron diffraction of adsorbed monolayers.
我们展示了二肉豆蔻酰磷脂酰乙醇胺（DMPE）双层膜的极性或头基区域的图像，该双层膜通过Langmuir-Blodgett沉积法在高表面压力下沉积在云母基板上，并在室温下于水中用光学杠杆原子力显微镜成像。DMPE的晶格结构以足够的分辨率可视化，从而可以确定单个头基的位置。作用力足够小，以至于同一区域可以在最小程度的损伤下重复成像。双层膜中的DMPE分子似乎具有相对良好的长程取向有序性，但短程和位置有序性较差。这些结果与水表面无支撑脂质单层的X射线测量结果以及吸附单层的电子衍射结果非常吻合。
