比较二甲基亚硝胺诱导的肝硬化大鼠模型脾切除和脾动脉结扎后肝脏再生情况。
Comparison of liver regeneration after a splenectomy and splenic artery ligation in a dimethylnitrosamine-induced cirrhotic rat model.
机构信息
Department of Surgery, Kurume University School of Medicine, 67 Asahi-machi, Kurume-city, Fukuoka, Japan.
出版信息
HPB (Oxford). 2010 Feb;12(1):22-30. doi: 10.1111/j.1477-2574.2009.00116.x.
AIM
A splenectomy and splenic artery ligation accelerate liver regeneration and improve liver function after a hepatectomy. However, there are no studies that directly compared the effects of a splenectomy and splenic artery ligation. In the present study, we compared the effects of a splenectomy and splenic artery ligation in cirrhotic rats.
METHODS
Dimethylnitrosamine (DMN) was administered intraperitoneally for 4 weeks to induce cirrhosis. The rats were divided into three groups: sham operation (CT group), splenic artery ligation (SAL group) and splenectomy (SP group). Liver functions [alanine aminotransferase (ALT) and total bilirubin (T. Bil)], plasma TGF-beta1, histopathological changes, extent of liver fibrosis (fibrotic rate) and regeneration [Ki-67 labelling index(LI)] were investigated in each group.
RESULTS
ALT and T. Bil levels were significantly lower in the SP group than the CT and SAL groups. TGF-beta1 levels were significantly lower in the SP group than in the CT and SAL groups. The fibrotic rate was significantly lower in the SP group than in the CT and SAL groups. The Ki-67 labelling index was significantly higher in the SP group than in the CT and SAL groups.
DISCUSSION
A splenectomy significantly improved liver regeneration with reduction of plasma TGF-beta1 levels compared with splenic artery ligation in DMN-treated cirrhotic rats.
目的
脾切除术和脾动脉结扎术可加速肝切除术后肝脏再生并改善肝功能。然而，目前尚无研究直接比较脾切除术和脾动脉结扎术的效果。在本研究中，我们比较了肝硬化大鼠脾切除术和脾动脉结扎术的效果。
方法
采用腹腔内注射二甲基亚硝胺（DMN）4 周诱导肝硬化。大鼠分为三组：假手术（CT 组）、脾动脉结扎（SAL 组）和脾切除术（SP 组）。检测各组肝功能[丙氨酸氨基转移酶（ALT）和总胆红素（T. Bil）]、血浆 TGF-β1、组织病理学变化、肝纤维化程度（纤维化率）和再生[Ki-67 标记指数（LI）]。
结果
SP 组的 ALT 和 T. Bil 水平明显低于 CT 和 SAL 组。SP 组的 TGF-β1 水平明显低于 CT 和 SAL 组。SP 组的纤维化率明显低于 CT 和 SAL 组。SP 组的 Ki-67 标记指数明显高于 CT 和 SAL 组。
讨论
与脾动脉结扎相比，DMN 处理的肝硬化大鼠脾切除术后，脾切除术可显著改善肝脏再生，降低血浆 TGF-β1 水平。
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