Coman L, Coman Oana Andreia, Păunescu H, Drăghia Fl, Fulga I
Department of Physiology, Faculty of Medicine, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania.
Rom J Morphol Embryol. 2010;51(2):327-36.
Various conditions may cause vascularization of the normally avascular cornea. The aim of the present study was to create a reproducible experimental model that could enable the investigation of the phenomena leading to corneal vascularization. This involved creating a software to record the experimental data, enabling a subsequent digital analysis based on the growth models. The VEGF-induced pattern of neovascularization was also investigated.
Twenty-seven rabbits divided in groups were used for the purposes of the present study. Some of them underwent intracorneal implants with or without vascular endothelial growth factor (VEGF) pellets, using an original microsurgical technique. Central and peripheral corneal burns were induced to other groups of animals in order to mimic the neovascularization process induced by inflammation. Finally, Dexamethasone (Maxidex) was given intraocularly, on days 1 and 3 after the onset of neovascularisation, in rabbit groups with both corneal burns and VEGF-implants. Video recording and data analysis of the corneal vascularization were made with an advanced biomicroscope, a computerized imaging system and a special software. A histochemical study of the animals' eyes was also carried out.
The recorded data showed the simplicity and reproducibility of the present experimental model. The results showed the importance of VEGF as an initiator and promoter of corneal vascularization through a non-inflammatory mechanism, quite different from the inflammation illustrated by the corneal burn. At the same time, Dexamethasone therapy proved its effectiveness in corneal angiogenesis induced by thermal burn, but not by VEGF-implant.
多种情况可能导致正常无血管的角膜出现血管化。本研究的目的是创建一个可重复的实验模型，以便能够研究导致角膜血管化的现象。这涉及创建一个软件来记录实验数据，从而基于生长模型进行后续的数字分析。还研究了血管内皮生长因子（VEGF）诱导的新生血管形成模式。
本研究使用了27只分组的兔子。其中一些兔子采用一种原创的显微手术技术进行角膜内植入，植入物有无血管内皮生长因子（VEGF）微丸。对其他几组动物进行角膜中央和周边烧伤，以模拟炎症诱导的新生血管形成过程。最后，在新生血管形成开始后的第1天和第3天，对既有角膜烧伤又有VEGF植入物的兔子组进行眼内给予地塞米松（Maxidex）。使用先进的生物显微镜、计算机成像系统和特殊软件对角膜血管化进行视频记录和数据分析。还对动物的眼睛进行了组织化学研究。
记录的数据显示了本实验模型的简易性和可重复性。结果表明，VEGF作为角膜血管化的启动剂和促进剂，通过一种非炎症机制发挥作用，这与角膜烧伤所显示的炎症情况截然不同。同时，地塞米松疗法在热烧伤诱导的角膜血管生成中证明了其有效性，但在VEGF植入诱导的角膜血管生成中则不然。
