Department of Chemistry, Graduate Center & The City University of New York, Brooklyn College, Brooklyn, NY, USA.
Photochem Photobiol. 2010 Jul-Aug;86(4):890-4. doi: 10.1111/j.1751-1097.2010.00748.x. Epub 2010 May 21.
Traditionally, Type II heterogeneous photo-oxidations produce singlet oxygen via external irradiation of a sensitizer and external supply of ground-state oxygen. A potential improvement is reported here. A hollow-core fiber-optic device was developed with an "internal" supply of light and flowing oxygen, and a porous photosensitizer-end capped configuration. Singlet oxygen was delivered through the fiber tip. The singlet oxygen steady-state concentration in the immediate vicinity of the probe tip was ca 20 fm by N-benzoyl-DL-methionine trapping. The device is portable and the singlet oxygen-generating tip is maneuverable, which opened the door to simple disinfectant studies. Complete Escherichia coli inactivation was observed in 2 h when the singlet oxygen sensitizing probe tip was immersed in 0.1 mL aqueous samples of 0.1-4.4 x 10(7) cells. Photobleaching of the probe tip occurred after ca 12 h of use, requiring baking and sensitizer reloading steps for reuse.
传统上，II 型异质光氧化通过敏化剂的外部照射和基态氧的外部供应来产生单线态氧。这里报道了一种潜在的改进方法。开发了一种具有“内部”光源和流动氧气供应以及多孔光敏剂端帽配置的中空光纤设备。单线态氧通过光纤尖端输送。通过 N-苯甲酰-DL-蛋氨酸捕获，探针尖端附近的单线态氧稳态浓度约为 20fm。该设备便携，产生单线态氧的探头可移动，这为简单的消毒剂研究开辟了道路。当将单线态氧敏化探头浸入 0.1 至 4.4 x 10(7)个细胞的 0.1 毫升水溶液样品中时，观察到大肠杆菌完全失活，需要在 12 小时的使用后进行探头的光漂白，需要进行烘焙和敏化剂重新加载步骤才能重复使用。
