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阿根廷东南部蚊子的分布及基于 18S rDNA 和 COI 序列分析的首次报告。

Distribution of mosquitoes in the south east of Argentina and first report on the analysis based on 18S rDNA and COI sequences.

机构信息

Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC), CONICET, Mar del Plata, Argentina.

出版信息

PLoS One. 2013 Sep 30;8(9):e75516. doi: 10.1371/journal.pone.0075516. eCollection 2013.

Abstract

Although Mar del Plata is the most important city on the Atlantic coast of Argentina, mosquitoes inhabiting such area are almost uncharacterized. To increase our knowledge in their distribution, we sampled specimens of natural populations. After the morphological identification based on taxonomic keys, sequences of DNA from small ribosomal subunit (18S rDNA) and cytochrome c oxidase I (COI) genes were obtained from native species and the phylogenetic analysis of these sequences were done. Fourteen species from the genera Uranotaenia, Culex, Ochlerotatus and Psorophora were found and identified. Our 18S rDNA and COI-based analysis indicates the relationships among groups at the supra-species level in concordance with mosquito taxonomy. The introduction and spread of vectors and diseases carried by them are not known in Mar del Plata, but some of the species found in this study were reported as pathogen vectors.

摘要

虽然马德普拉塔是阿根廷大西洋海岸最重要的城市,但栖息在该地区的蚊子几乎没有特征。为了增加我们对它们分布的了解,我们对自然种群的标本进行了采样。在基于分类学关键的形态学鉴定之后,从小核糖体亚基(18S rDNA)和细胞色素 c 氧化酶 I(COI)基因中获得了来自本地物种的 DNA 序列,并对这些序列进行了系统发育分析。从 Uranotaenia、Culex、Ochlerotatus 和 Psorophora 属中发现并鉴定了 14 种蚊子。我们基于 18S rDNA 和 COI 的分析表明,在超种水平上的群体之间的关系与蚊子分类学一致。马德普拉塔市不知道媒介和携带的疾病的传入和传播,但本研究中发现的一些物种被报道为病原体媒介。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bb4/3787072/f28de0267589/pone.0075516.g001.jpg

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