槲皮素诱导离体大鼠肝细胞核的脂质过氧化和DNA损伤。

Quercetin-induced lipid peroxidation and DNA damage in isolated rat-liver nuclei.

作者信息

Sahu S C, Washington M C

机构信息

Molecular Toxicology Laboratory, Food and Drug Administration, Washington, DC 20204.

出版信息

Cancer Lett. 1991 Jun 14;58(1-2):75-9. doi: 10.1016/0304-3835(91)90026-e.

DOI:10.1016/0304-3835(91)90026-e

PMID:2049786

Abstract

The extent of lipid peroxidation and DNA damage induced by quercetin were studied under aerobic conditions in isolated rat-liver nuclei. The effects of iron and copper ions on these two toxic oxidative processes were also investigated. Quercetin induced significant (P less than 0.05) concentration-dependent nuclear lipid peroxidation concurrent with DNA degradation; these effects were enhanced by iron and copper ions. The results suggest that the reactive oxygen species generated by quercetin autoxidation, catalyzed by iron and copper ions, are responsible for the concurrent lipid peroxidation and DNA damage in isolated rat-liver nuclei.

摘要

在有氧条件下，对分离出的大鼠肝细胞核中槲皮素诱导的脂质过氧化和DNA损伤程度进行了研究。还研究了铁离子和铜离子对这两个毒性氧化过程的影响。槲皮素诱导了显著的（P小于0.05）浓度依赖性核脂质过氧化，并伴有DNA降解；铁离子和铜离子增强了这些作用。结果表明，由铁离子和铜离子催化的槲皮素自氧化产生的活性氧，是分离出的大鼠肝细胞核中脂质过氧化和DNA损伤同时发生的原因。