Department of Medicine, Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.
Lab Invest. 2010 Sep;90(9):1274-84. doi: 10.1038/labinvest.2010.104. Epub 2010 May 24.
Serum response factor (SRF) is a ubiquitously expressed transcription factor that binds to a DNA cis element known as the CArG box, which is found in the proximal regulatory regions of over 200 experimentally validated target genes. Genetic deletion of SRF is incompatible with life in a variety of animals from different phyla. In mice, loss of SRF throughout the early embryo results in gastrulation defects precluding analyses in individual organ systems. Genetic inactivation studies using conditional or inducible promoters directing the expression of the bacteriophage Cre recombinase have shown a vital role for SRF in such cellular processes as contractility, cell migration, synaptic activity, inflammation, and cell survival. A growing number of experimental and human diseases are associated with changes in SRF expression, suggesting that SRF has a role in the pathogenesis of disease. This review summarizes data from experimental model systems and human pathology where SRF expression is either deliberately or naturally altered.
血清应答因子 (SRF) 是一种广泛表达的转录因子，它与一种称为 CArG 盒的 DNA 顺式元件结合，该元件存在于 200 多个经过实验验证的靶基因的近端调控区域。从不同门的各种动物中删除 SRF 与生命是不相容的。在小鼠中，整个早期胚胎中 SRF 的缺失导致原肠胚形成缺陷，从而排除了对单个器官系统的分析。使用条件性或诱导性启动子指导噬菌体 Cre 重组酶表达的基因失活研究表明，SRF 在收缩性、细胞迁移、突触活动、炎症和细胞存活等细胞过程中具有重要作用。越来越多的实验和人类疾病与 SRF 表达的变化有关，这表明 SRF 在疾病的发病机制中起作用。这篇综述总结了来自实验模型系统和人类病理学的数据，其中 SRF 的表达要么是故意改变的，要么是自然改变的。
