Hildebrandt M, Saur U, Nellen W
Max-Planck-Institut f. Biochemie, Abt. Zellbiologie, Martinsried, Federal Republic of Germany.
Dev Genet. 1991;12(1-2):163-9. doi: 10.1002/dvg.1020120125.
We present the nucleotide sequence of the cell type specific prespore gene EB4 which encodes a protein containing a hydrophobic leader sequence and two distinct domains of amino acid repeats. By RNase protection experiments we have determined the genomic organization of the gene which differs substantially from the previously published data. An antibody directed against one of the repeat structures (hexamer repeat) specifically reacts with a developmentally regulated antigen of 58 kd. Gene disruption transformants were obtained by transformation with a genomic DNA fragment. The transformants express a 3' truncated mRNA and do not react with the anti-hexamer antibody. So far, we could not detect any phenotypic aberrations in the transformed cell line.
我们展示了细胞类型特异性前孢子基因EB4的核苷酸序列，该基因编码一种含有疏水前导序列和两个不同氨基酸重复结构域的蛋白质。通过核糖核酸酶保护实验，我们确定了该基因的基因组结构，其与先前发表的数据有很大差异。一种针对其中一个重复结构（六聚体重复）的抗体与一种58kd的发育调控抗原特异性反应。通过用基因组DNA片段转化获得了基因破坏转化体。这些转化体表达一种3'端截短的mRNA，并且不与抗六聚体抗体反应。到目前为止，我们在转化的细胞系中未检测到任何表型异常。
