Faculty of Arts and Sciences, Department of Chemistry, Trakya University, 22030, Edirne, Turkey.
Environ Monit Assess. 2011 Apr;175(1-4):303-14. doi: 10.1007/s10661-010-1513-5. Epub 2010 May 25.
In this study, soil samples were collected from Edirne, Turkey in both summer and winter seasons and subjected to the modified Community Bureau of Reference (BCR) sequential extraction procedure in order to investigate the chemical partitioning of metals in soils and to predict heavy metals uptake by wheat grains which grown at the same soils. The samples were subjected to a three stage extraction procedure proposed by the BCR. The three phases that were separated out in the following order: (1) carbonate, exchangeable, (2) Fe-Mn oxides, and (3) organic matter. Metal concentrations of soil fractions and grain samples were determined by inductively coupled plasma atomic emission spectroscopy. The wheat samples were prepared to analysis using microwave acid digestion procedure. The pseudo-total concentrations of metals were determined after aqua regia digestion. The analytical accuracy of the method was evaluated by using the Standard Reference Materials (BCR 142R Light Sandy Soil, NIST 2711 Montana Soil, and NIST 2704 Buffalo River Sediment). The sum of the metal contents obtained from the modified BCR sequential extraction procedure and pseudo-total metal contents for soil samples were used to calculate recovery values. In order to evaluate the bioavailability of metals, the relationships between the wheat-metal and soil-extractable metal concentrations were compared.
在这项研究中，从土耳其的埃迪尔内采集了土壤样本，分别在夏季和冬季进行了改良的社区参考机构（BCR）连续提取程序，以研究土壤中金属的化学分配情况，并预测在相同土壤中生长的小麦对重金属的吸收情况。样品经过 BCR 提出的三阶段提取程序处理。分离出的三个阶段顺序为：（1）碳酸盐、可交换，（2）铁-锰氧化物，（3）有机物。土壤部分和谷物样品中的金属浓度通过电感耦合等离子体原子发射光谱法确定。小麦样品采用微波酸消解法制备分析。使用王水消解后测定金属的全量浓度。通过使用标准参考物质（BCR 142R 轻质沙壤土、NIST 2711 蒙大拿州土壤和 NIST 2704 布法罗河沉积物）评估方法的分析准确性。从改良的 BCR 连续提取程序和土壤样品的全量金属含量中获得的金属含量总和用于计算回收值。为了评估金属的生物利用度，比较了小麦-金属和土壤可提取金属浓度之间的关系。
